ArticleProceedings of the National Academy of Sciences of the United States of America2007
High-resolution analysis of Drosophila heterochromatin organization using SuUR Su(var)3-9 double mutants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
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- Dispersive forces and resisting spot welds by alternative homolog conjunction govern chromosome shape in Drosophila spermatocytes during prophase I.PLoS genetics · 2022Article
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- Effects of Mutations in theCells · 2020Article
- Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster.Chromosoma · 2019Article
- Genetic and Molecular Analysis of Essential Genes in Centromeric Heterochromatin of the Left Arm of Chromosome 3 inG3 (Bethesda, Md.) · 2019Article
- Islands of retroelements are major components of Drosophila centromeres.PLoS biology · 2019Article
- Simple and Complex Centromeric Satellites inGenetics · 2018Article
- Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication inGenes & development · 2017Article
- The structure of an endogenous Drosophila centromere reveals the prevalence of tandemly repeated sequences able to form i-motifs.Scientific reports · 2015Article
- The Release 6 reference sequence of the Drosophila melanogaster genome.Genome research · 2015Article
- Homolog pairing and sister chromatid cohesion in heterochromatin in Drosophila male meiosis I.Chromosoma · 2011Article
- The SUUR protein is involved in binding of SU(VAR)3-9 and methylation of H3K9 and H3K27 in chromosomes of Drosophila melanogaster.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
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- Heterochromatic genome stability requires regulators of histone H3 K9 methylation.PLoS genetics · 2009Article
- Review
- Local DNA underreplication correlates with accumulation of phosphorylated H2Av in the Drosophila melanogaster polytene chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structural and functional analyses of heterochromatin are essential to understanding how heterochromatic genes are regulated and how centromeric chromatin is formed. Because the repetitive nature of heterochromatin hampers its genome analysis, new approaches need to be developed. Here, we describe how, in double mutants for Su(var)3-9 and SuUR genes encoding two structural proteins of heterochromatin, new banded heterochromatic segments appear in all polytene chromosomes due to the strong suppression of under-replication in pericentric regions. FISH on salivary gland polytene chromosomes from these double mutant larvae allows high resolution of heterochromatin mapping. In addition, immunostaining experiments with a set of antibodies against euchromatic and heterochromatic proteins reveal their unusual combinations in the newly appeared segments: binding patterns for HP1 and HP2 are coincident, but both are distinct from H3diMetK9 and H4triMetK20. In several regions, partial overlapping staining is observed for the proteins characteristic of active chromatin RNA Pol II, H3triMetK4, Z4, and JIL1, the boundary protein BEAF, and the heterochromatin-enriched proteins HP1, HP2, and SU(VAR)3-7. The exact cytological position of the centromere of chromosome 3 was visualized on salivary gland polytene chromosomes by using the centromeric dodeca satellite and the centromeric protein CID. This region is enriched in H3diMetK9 and H4triMetK20 but is devoid of other proteins analyzed.
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