ArticleJournal of molecular biology2007
Structural analysis and dynamics of retinal chromophore in dark and meta I states of rhodopsin from 2H NMR of aligned membranes.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 54 citations in OpenAlex.
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Synthesis of 9-CDTetrahedron letters · 2018Article
- Static solid-stateProgress in nuclear magnetic resonance spectroscopy · 2017Review
- Article
- CONDENSED-MATTER SPECTROSCOPY SPECTRAL METHODS FOR STUDY OF THE G-PROTEIN-COUPLED RECEPTOR RHODOPSIN. II. MAGNETIC RESONANCE METHODS.Optics and spectroscopy · 2016Article
- Retinal Conformation Changes Rhodopsin's Dynamic Ensemble.Biophysical journal · 2015Article
- SPECTRAL METHODS FOR STUDY OF THE G-PROTEIN-COUPLED RECEPTOR RHODOPSIN. I. VIBRATIONAL AND ELECTRONIC SPECTROSCOPY.Optics and spectroscopy · 2015Article
- Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Article
- Investigation of rhodopsin dynamics in its signaling state by solid-state deuterium NMR spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Amino acid conservation and interactions in rhodopsin: probing receptor activation by NMR spectroscopy.Biochimica et biophysica acta · 2014Review
- Retinal ligand mobility explains internal hydration and reconciles active rhodopsin structures.Biochemistry · 2014Article
- Deconstructing activation events in rhodopsin.Journal of the American Chemical Society · 2013Article
- Molecular simulations and solid-state NMR investigate dynamical structure in rhodopsin activation.Biochimica et biophysica acta · 2012Review
- Solid-State NMR of a Large Membrane Protein by Paramagnetic Relaxation Enhancement.The journal of physical chemistry letters · 2011Article
- Solid-state 2H NMR relaxation illuminates functional dynamics of retinal cofactor in membrane activation of rhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Solid-state NMR study of the charge-transfer complex between ubiquinone-8 and disulfide bond generating membrane protein DsbB.Journal of the American Chemical Society · 2011Article
- Retinal dynamics underlie its switch from inverse agonist to agonist during rhodopsin activation.Nature structural & molecular biology · 2011Article
- Anabaena sensory rhodopsin is a light-driven unidirectional rotor.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Structure and function of G protein-coupled receptors using NMR spectroscopy.Progress in nuclear magnetic resonance spectroscopy · 2010Review
- Sequential rearrangement of interhelical networks upon rhodopsin activation in membranes: the Meta II(a) conformational substate.Journal of the American Chemical Society · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Rhodopsin is a prototype for G protein-coupled receptors (GPCRs) that are implicated in many biological responses in humans. A site-directed (2)H NMR approach was used for structural analysis of retinal within its binding cavity in the dark and pre-activated meta I states. Retinal was labeled with (2)H at the C5, C9, or C13 methyl groups by total synthesis, and was used to regenerate the opsin apoprotein. Solid-state (2)H NMR spectra were acquired for aligned membranes in the low-temperature lipid gel phase versus the tilt angle to the magnetic field. Data reduction assumed a static uniaxial distribution, and gave the retinylidene methyl bond orientations plus the alignment disorder (mosaic spread). The dark-state (2)H NMR structure of 11-cis-retinal shows torsional twisting of the polyene chain and the beta-ionone ring. The ligand undergoes restricted motion, as evinced by order parameters of approximately 0.9 for the spinning C-C(2)H(3) groups, with off-axial fluctuations of approximately 15 degrees . Retinal is accommodated within the rhodopsin binding pocket with a negative pre-twist about the C11=C12 double bond that explains its rapid photochemistry and the trajectory of 11-cis to trans isomerization. In the cryo-trapped meta I state, the (2)H NMR structure shows a reduction of the polyene strain, while torsional twisting of the beta-ionone ring is maintained. Distortion of the retinal conformation is interpreted through substituent control of receptor activation. Steric hindrance between trans retinal and Trp265 can trigger formation of the subsequent activated meta II state. Our results are pertinent to quantum and molecular mechanics simulations of ligands bound to GPCRs, and illustrate how (2)H NMR can be applied to study their biological mechanisms of action.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.