ArticleThe Biochemical journal1991
Clonal analysis of sucrase-isomaltase expression in the human colon adenocarcinoma Caco-2 cells.
Article in The Biochemical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 150 citations in OpenAlex.
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- Src family kinases inhibit differentiation of intestinal epithelial cells through the Hippo effector YAP1.Biology open · 2021Article
- Peroxidized Linoleic Acid, 13-HPODE, Alters Gene Expression Profile in Intestinal Epithelial Cells.Foods (Basel, Switzerland) · 2021Article
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- The In Vitro Protective Role of Bovine Lactoferrin on Intestinal Epithelial Barrier.Molecules (Basel, Switzerland) · 2019Article
- MYC Regulates α6 Integrin Subunit Expression and Splicing Under Its Pro-Proliferative ITGA6A Form in Colorectal Cancer Cells.Cancers · 2018Article
- Involvement of CRF2 signaling in enterocyte differentiation.World journal of gastroenterology · 2017Article
- Epigenetics in Intestinal Epithelial Cell Renewal.Journal of cellular physiology · 2016Review
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- Histone deacetylase inhibition impairs normal intestinal cell proliferation and promotes specific gene expression.Journal of cellular biochemistry · 2015Article
- Altered intestinal functions and increased local inflammation in insulin-resistant obese subjects: a gene-expression profile analysis.BMC gastroenterology · 2015Article
- The calcium-sensing receptor: A promising target for prevention of colorectal cancer.Biochimica et biophysica acta · 2015Article
- Regulation of the calcium-sensing receptor expression by 1,25-dihydroxyvitamin D3, interleukin-6, and tumor necrosis factor alpha in colon cancer cells.The Journal of steroid biochemistry and molecular biology · 2014Review
- A gene expression programme induced by bovine colostrum whey promotes growth and wound-healing processes in intestinal epithelial cells.Journal of nutritional science · 2014Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Insights into the pathogenesis of enteropathogenic E. coli using an improved intestinal enterocyte model.PloS one · 2013Article
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- Polycomb repressive complex 2 impedes intestinal cell terminal differentiation.Journal of cell science · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the biosynthetic basis for the mosaic expression of brush border enzymes in confluent Caco-2 cells, a human colon carcinoma cell line exhibiting characteristics of adult small intestinal enterocytes, we have obtained a series of clones differing markedly in their growth rates, amounts of transforming growth factor-alpha/epidermal growth factor-like activity released into the culture medium, and sucrase-isomaltase (SI) activity. Other intestinal markers (aminopeptidase N, dipeptidylpeptidase IV, lactase, alkaline phosphatase and 'crypt cell antigen') displayed a much more limited variability in expression, suggesting that the Caco-2 cell clones we have obtained did not differ in their overall ability to differentiate. Immunofluorescence staining, metabolic labelling with radioactive methionine and hybridization analysis of SI mRNA abundance were used to investigate SI synthesis and its regulation in clones endowed with low, intermediate or high sucrase activity. The results obtained have demonstrated heterogeneous SI expression, even in clonal cell lines, and a negative correlation between SI expression and growth factor concentrations in the culture medium, suggesting an autocrine regulation of cell proliferation and differentiation in confluent Caco-2 cells. Pulse-chase experiments using the two clones endowed with the lowest and highest levels of SI activity, followed by immunoprecipitation of labelled SI with epitope-specific antibodies and SDS/PAGE analysis, suggested that both transcriptional and post-translational mechanisms play a role in the regulation of SI expression in intestinal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.