ArticleAntimicrobial agents and chemotherapy2007
Cloning, nucleotide sequencing, and analysis of the AcrAB-TolC efflux pump of Enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolate.
Article in Antimicrobial agents and chemotherapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Mutational analysis of multidrug efflux pump (AcrAB-TolC) in Enterobacter cloacae isolates from clinical samples.Scientific reports · 2026Article
- Effect of sub-inhibitory tigecycline (½-MIC) on AcrAB-TolC andFrontiers in cellular and infection microbiology · 2026Article
- The Effect of the PhoP/PhoQ System on the Regulation of Multi-Stress Adaptation Induced by Acid Stress inFoods (Basel, Switzerland) · 2024Article
- Paradoxical Activation of a Type VI Secretion System Phospholipase Effector by Its Cognate Immunity Protein.Journal of bacteriology · 2023Article
- A comprehensive description of the TolC effect on the antimicrobial susceptibility profile inFrontiers in cellular and infection microbiology · 2022Article
- Potentiating antibiotic efficacy via perturbation of non-essential gene expression.Communications biology · 2021Article
- Review
- Detection and characterization of tigecycline heteroresistance in E. cloacae: clinical and microbiological findings.Emerging microbes & infections · 2019Article
- CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance.Frontiers in bioengineering and biotechnology · 2018Article
- Virulence and Antibiotic Resistance Profiles ofFrontiers in microbiology · 2018Article
- Article
- Structural Alteration of OmpR as a Source of Ertapenem Resistance in a CTX-M-15-Producing Escherichia coli O25b:H4 Sequence Type 131 Clinical Isolate.Antimicrobial agents and chemotherapy · 2017Article
- Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil.BMC microbiology · 2017Article
- Diminution of the gut resistome after a gut microbiota-targeted dietary intervention in obese children.Scientific reports · 2016Article
- Landscape of Resistance-Nodulation-Cell Division (RND)-Type Efflux Pumps in Enterobacter cloacae Complex.Antimicrobial agents and chemotherapy · 2016Article
- Molecular Characterization of Carbapenem-Nonsusceptible Enterobacterial Isolates Collected during a Prospective Interregional Survey in France and Susceptibility to the Novel Ceftazidime-Avibactam and Aztreonam-Avibactam Combinations.Antimicrobial agents and chemotherapy · 2016Article
- Altered Outer Membrane Transcriptome Balance with AmpC Overexpression in Carbapenem-ResistantFrontiers in microbiology · 2016Article
- Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment.Frontiers in microbiology · 2015Review
- CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria.Structure (London, England : 1993) · 2014Article
- Antimicrobial resistance and virulence: a successful or deleterious association in the bacterial world?Clinical microbiology reviews · 2013Review
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. Management of these infections is often difficult, owing to the high frequency of strains that are resistant to disinfectants and antimicrobial agents in the clinical setting. Multidrug efflux pumps, especially those belonging to the resistance-nodulation-division family, play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. In the present study, we cloned and sequenced the genes encoding an AcrAcB-TolC-like efflux pump from an E. cloacae clinical isolate (isolate EcDC64) showing a broad antibiotic resistance profile. Sequence analysis showed that the acrR, acrA, acrB, and tolC genes encode proteins that display 79.8%, 84%, 88%, and 82% amino acid identities with the respective homologues of Enterobacter aerogenes and are arranged in a similar pattern. Deletion of the acrA gene to yield an AcrA-deficient EcDC64 mutant (EcDeltaacrA) showed the involvement of AcrAB-TolC in multidrug resistance in E. cloacae. However, experiments with an efflux pump inhibitor suggested that additional efflux systems also play a role in antibiotic resistance. Investigation of several unrelated isolates of E. cloacae by PCR analysis revealed that the AcrAB system is apparently ubiquitous in this species.
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