ArticleTransgenic research2008
Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Breaking transformation barriers.Nature plants · 2023Article
- Comparison of the impact of viral and plant-derived promoters regulating selectable marker gene on maize transformation and transgene expression.Plant cell reports · 2017Article
- Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains.Transgenic research · 2017Article
- Screening Auxin Response, In Vitro Culture Aptitude and Susceptibility to Agrobacterium-Mediated Transformation of Italian Commercial Durum Wheat Varieties.Molecules (Basel, Switzerland) · 2016Article
- Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens.Genetics and molecular biology · 2015Article
- High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection.Protoplasma · 2015Article
- Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants.Transgenic research · 2013Article
- Article
- Increasing the amylose content of durum wheat through silencing of the SBEIIa genes.BMC plant biology · 2010Article
- Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency.Journal of experimental botany · 2010Article
- Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens.Plant cell reports · 2009Article
- Transgenic cowpea (Vigna unguiculata) seeds expressing a bean alpha-amylase inhibitor 1 confer resistance to storage pests, bruchid beetles.Plant cell reports · 2008Article
- Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins.Transgenic research · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Genetic transformation of wheat, using biolistics or Agrobacterium, underpins a range of specific research methods for identifying genes and studying their function in planta. Transgenic approaches to study and modify traits in durum wheat have lagged behind those for bread wheat. Here we report the use of Agrobacterium strain AGL1, with additional vir genes housed in a helper plasmid, to transform and regenerate the durum wheat variety Ofanto. The use of the basic pSoup helper plasmid with no additional vir genes failed to generate transformants, whereas the presence of either virG542 or the 15 kb Komari fragment containing virB, virC and virG542 produced transformation efficiencies of between 0.6 and 9.7%. Of the 42 transgenic plants made, all but one (which set very few seeds) appeared morphologically normal and produced between 100 and 300 viable seeds. The transgene copy number and the segregation ratios were found to be very similar to those previously reported for bread wheat. We believe that this is the first report describing successful genetic transformation of tetraploid durum wheat (Triticum turgidum L. var. durum) mediated by Agrobacterium tumefaciens using immature embryos as the explant.
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