ArticlePlant cell reports2007
Direct creation of marker-free tobacco plants from agroinfiltrated leaf discs.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed.
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Consequences of adaptation of TAL effectors on host susceptibility to Xanthomonas.PLoS genetics · 2021Article
- Genome-wide analysis of water-stress-responsive microRNA expression profile in tobacco roots.Functional & integrative genomics · 2014Article
- Targeted genome editing of sweet orange using Cas9/sgRNA.PloS one · 2014Article
- Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata.Plant cell reports · 2012Article
- Assessment of simple marker-free genetic transformation techniques in alfalfa.Plant cell reports · 2011Article
- An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.).Plant cell reports · 2010Article
- Article
- Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency.Plant cell reports · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agroinfiltration is employed as a fast way to directly create marker-free transgenic tobacco plants. As an example for the efficiency of the method, Agrobacterium cells harboring a marker-free vector coding for beta-glucuronidase (GUS) were infiltrated into the leaf discs of Nicotiana tabacum, which were then used as explants for marker-free plant regeneration by tissue culture. Through GUS staining, a large number of small calli were shown to be stably transformed on the treated leaf discs at 17 days after agroinfiltration. Most importantly, after continuous culture of the leaf discs until shoot regeneration, about 15% of the regenerants were proven to be transformants by polymerase chain reaction (PCR) analysis.
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Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.