ArticlePloS one2007
Assembly of microtubules and actomyosin rings in the absence of nuclei and spindle pole bodies revealed by a novel genetic method.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 6 citations in OpenAlex.
- Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans.The Journal of cell biology · 2026Article
- Opposing kinesin complexes queue at plus tips to ensure microtubule catastrophe at cell ends.EMBO reports · 2018Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe nucleus and the centrosomes (spindle pole bodies; SPBs in yeast) are believed to play key roles in the organization of various cellular structures, such as the actomyosin ring and microtubules. The ability to generate cells lacking nuclei and centrosomes (SPBs) is key to the elucidation of the role of these structures in various cellular processes. METHODOLOGY/PRINCIPAL
findingsHere we describe a genetic method, using the Schizosaccharomyces pombe cdc16-116 mutant, to reliably and efficiently generate fission yeast cells lacking nuclei and SPBs. We use this approach to show that the assembly of microtubules does not require nuclear associated microtubule organizing centers and SPBs. We also show that actomyosin rings can assemble albeit inefficiently in the absence of nuclei and SPBs.
conclusionWe conclude that key cytoskeletal elements can be assembled in the absence of nuclei and SPBs. In addition, the approach we describe, taken together with physical approaches such as centrifugation, should facilitate the investigation of the role of the nucleus and SPBs in the assembly and inheritance of various cellular structures and organelles.
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Registered trials
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