ArticleGene therapy2007
Baculovirus as a new gene delivery vector for stem cell engineering and bone tissue engineering.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 73 citations in OpenAlex.
- Advances in Genetic Reprogramming: Prospects from Developmental Biology to Regenerative Medicine.Current medicinal chemistry · 2024Review
- Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium.Stem cell research & therapy · 2016Article
- Control of stem cell fate by engineering their micro and nanoenvironment.World journal of stem cells · 2015Review
- Bone formation in rabbit's leg muscle after autologous transplantation of bone marrow-derived mesenchymal stem cells expressing human bone morphogenic protein-2.Indian journal of orthopaedics · 2014Article
- Modulation of osteoblastic/odontoblastic differentiation of adult mesenchymal stem cells through gene introduction: a brief review.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2013Review
- Optimal construction and delivery of dual-functioning lentiviral vectors for type I collagen-suppressed chondrogenesis in synovium-derived mesenchymal stem cells.Pharmaceutical research · 2011Article
- Baculovirus expression systems for production of recombinant proteins in insect and mammalian cells.Molecular biology · 2011Article
- Baculovirus-transduced bone marrow mesenchymal stem cells for systemic cancer therapy.Cancer gene therapy · 2010Article
- Screening of complement inhibitors: shielded baculoviruses increase the safety and efficacy of gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway.Journal of virology · 2009Article
- Purification of recombinant baculoviruses for gene therapy using membrane processes.Gene therapy · 2009Article
- Improved adenovirus type 5 vector-mediated transduction of resistant cells by piggybacking on coxsackie B-adenovirus receptor-pseudotyped baculovirus.Journal of virology · 2009Article
- Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Development of a hybrid baculoviral vector for sustained transgene expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- HSV-1 amplicon viral vector-mediated gene transfer to human bone marrow-derived mesenchymal stem cells.Cancer gene therapy · 2008Article
- Baculovirus as a gene delivery vector: recent understandings of molecular alterations in transduced cells and latest applications.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Baculovirus has emerged as a novel vector for in vitro and in vivo gene delivery due to its low cytotoxicity and non-replication nature in mammalian cells, but the applications of baculovirus in the genetic modification of human mesenchymal stem cells (hMSCs) and tissue engineering are yet to be reported. In this study, we genetically engineered hMSCs with a baculovirus (Bac-CB) expressing bone morphogenetic protein-2 (BMP-2). Bac-CB transduction of hMSCs at a multiplicity of infection of 40 triggered effective differentiation of hMSCs into osteoblasts. Supertransduction at day 6 after initial transduction enhanced the BMP-2 expression and further accelerated the in vitro osteogenesis, as confirmed by alkaline phosphatase assay, Alizarin red staining and reverse transcription-polymerase chain reaction analysis of osteoblastic genes. Implantation of the supertransduced cells at ectopic sites in the nude mice resulted in efficient cell differentiation into osteoblasts at week 2 and induced progressive mineralization and partial bone formation at week 6, as confirmed by hematoxylin and eosin, immunohistochemical and Alizarin red staining. These data collectively demonstrated, for the first time, the potential of baculovirus in hMSCs engineering and implicated its use in bone tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.