ArticleStructure (London, England : 1993)2007
A general strategy to solve the phase problem in RNA crystallography.
Article in Structure (London, England : 1993), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Evolution of ribosome X-ray crystallography: from first crystals to atomic resolution.Biophysical reviews · 2025Review
- A general strategy for engineering GU base pairs to facilitate RNA crystallization.Nucleic acids research · 2025Article
- sincFold: end-to-end learning of short- and long-range interactions in RNA secondary structure.Briefings in bioinformatics · 2024Article
- RNA structure determination: From 2D to 3D.Fundamental research · 2023Review
- General Strategies for RNA X-ray Crystallography.Molecules (Basel, Switzerland) · 2023Review
- Nearest neighbor rules for RNA helix folding thermodynamics: improved end effects.Nucleic acids research · 2022Article
- Review
- Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.Scientific reports · 2021Article
- Three-dimensional structure of a flavivirus dumbbell RNA reveals molecular details of an RNA regulator of replication.Nucleic acids research · 2021Article
- Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.Nature communications · 2021Article
- Structural Insights Into the 5'UG/3'GU Wobble Tandem in Complex With BaFrontiers in molecular biosciences · 2021Article
- The crystal structure of a Polerovirus exoribonuclease-resistant RNA shows how diverse sequences are integrated into a conserved fold.RNA (New York, N.Y.) · 2020Article
- Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.Molecules (Basel, Switzerland) · 2020Review
- Structure of an RNA helix with pyrimidine mismatches and cross-strand stacking.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Crystal structure of an adenovirus virus-associated RNA.Nature communications · 2019Article
- Nuclear Magnetic Resonance Reveals That GU Base Pairs Flanking Internal Loops Can Adopt Diverse Structures.Biochemistry · 2019Article
- Article
- Ribosome-induced RNA conformational changes in a viral 3'-UTR sense and regulate translation levels.Nature communications · 2018Article
- Structural Basis for Ligand Binding to the Guanidine-I Riboswitch.Structure (London, England : 1993) · 2017Article
- Applications of PLOR in labeling large RNAs at specific sites.Methods (San Diego, Calif.) · 2016Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.