ArticleProceedings of the National Academy of Sciences of the United States of America2007
FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
126 citing papers in PubMed, 321 citations in OpenAlex.
- A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.Journal of experimental botany · 2026Article
- Article
- Article
- Unraveling the role of OsPIP1;3 in arsenic transport in rice (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.Journal of experimental botany · 2025Article
- Genome-wide identification of Morus notabilis Aquaporin gene family and differential expression of plasma membrane intrinsic proteins in response to Ralstonia pseudosolanacearum infection.BMC plant biology · 2025Article
- Tissue-specific accumulation of PIP aquaporins of a particular heteromeric composition is part of the maize response to mycorrhiza and drought.Scientific reports · 2024Article
- PbHsfC1a-coordinates ABA biosynthesis and HPlant biotechnology journal · 2024Article
- Plasma membrane intrinsic protein OsPIP2;6 is involved in root-to-shoot arsenic translocation in rice (Oryza sativa L.).Plant cell reports · 2024Article
- Cooperativity in regulation of membrane protein function: phenomenological analysis of the effects of pH and phospholipids.Biophysical reviews · 2023Review
- Natural variation of maize root hydraulic architecture underlies highly diverse water uptake capacities.Plant physiology · 2023Article
- Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress.Plant physiology · 2023Article
- Subcellular positioning during cell division and cell plate formation in maize.Frontiers in plant science · 2023Article
- Exploring aquaporin functions during changes in leaf water potential.Frontiers in plant science · 2023Review
- Plasma membrane aquaporins of the PIP1 and PIP2 subfamilies facilitate hydrogen peroxide diffusion into plant roots.BMC plant biology · 2022Article
- Aquaporin Gating: A New Twist to Unravel Permeation through Water Channels.International journal of molecular sciences · 2022Review
- Using the Maize Nested Association Mapping (NAM) Population to Partition Arbuscular Mycorrhizal Effects on Drought Stress Tolerance into Hormonal and Hydraulic Components.International journal of molecular sciences · 2022Article
- OsHIPL1, a hedgehog-interacting protein-like 1 protein, increases seed vigour in rice.Plant biotechnology journal · 2022Article
- Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize.Journal of nanobiotechnology · 2022Article
- Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots.Antioxidants (Basel, Switzerland) · 2022Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zea mays plasma membrane intrinsic proteins (ZmPIPs) fall into two groups, ZmPIP1s and ZmPIP2s, that exhibit different water channel activities when expressed in Xenopus oocytes. ZmPIP1s are inactive, whereas ZmPIP2s induce a marked increase in the membrane osmotic water permeability coefficient, P(f). We previously showed that, in Xenopus oocytes, ZmPIP1;2 and ZmPIP2;1 interact to increase the cell P(f). Here, we report the localization and interaction of ZmPIP1s and ZmPIP2s in living maize cells. ZmPIPs were fused to monomeric yellow fluorescent protein and/or monomeric cyan fluorescent protein and expressed transiently in maize mesophyll protoplasts. When expressed alone, ZmPIP1 fusion proteins were retained in the endoplasmic reticulum, whereas ZmPIP2s were found in the plasma membrane. Interestingly, when coexpressed with ZmPIP2s, ZmPIP1s were relocalized to the plasma membrane. Using FRET/fluorescence lifetime imaging microscopy, we demonstrated that this relocalization results from interaction between ZmPIP1s and ZmPIP2s. Immunoprecipitation experiments provided additional evidence for the association of ZmPIP1;2 and ZmPIP2;1 in maize roots and suspension cells. These data suggest that PIP1-PIP2 interaction is required for in planta PIP1 trafficking to the plasma membrane to modulate plasma membrane permeability.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.