ArticleMolecular and cellular biology2007
Epigenetic activation of the human growth hormone gene cluster during placental cytotrophoblast differentiation.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Long-range looping of a locus control region drives tissue-specific chromatin packing within a multigene cluster.Nucleic acids research · 2016Article
- Expression of Placental Members of the Human Growth Hormone Gene Family Is Increased in Response to Sequential Inhibition of DNA Methylation and Histone Deacetylation.BioResearch open access · 2015Article
- Tissue specific CTCF occupancy and boundary function at the human growth hormone locus.Nucleic acids research · 2014Article
- CCAAT-enhancer-binding protein β (C/EBPβ) and downstream human placental growth hormone genes are targets for dysregulation in pregnancies complicated by maternal obesity.The Journal of biological chemistry · 2013Article
- Identification of a secondary promoter within the human B cell receptor component gene hCD79b.The Journal of biological chemistry · 2013Article
- Epigenetic modifications unlock the milk protein gene loci during mouse mammary gland development and differentiation.PloS one · 2013Article
- PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins.PloS one · 2013Article
- Bridging sequence diversity and tissue-specific expression by DNA methylation in genes of the mouse prolactin superfamily.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Article
- Differential placental expression profile of human Growth Hormone/Chorionic Somatomammotropin genes in pregnancies with pre-eclampsia and gestational diabetes mellitus.Molecular and cellular endocrinology · 2012Article
- Review
- Lactogens and estrogens in breast cancer chemoresistance.Expert review of endocrinology & metabolism · 2011Article
- Research resource: T-antigen transformation of pituitary cells captures three novel cell lines in the Pit-1 lineage.Molecular endocrinology (Baltimore, Md.) · 2010Article
- New insights into the regulation of human cytotrophoblast cell differentiation.Molecular and cellular endocrinology · 2010Review
- Appearance of the pituitary factor Pit-1 increases chromatin remodeling at hypersensitive site III in the human GH locus.Journal of molecular endocrinology · 2010Article
- Differential expression profile of growth hormone/chorionic somatomammotropin genes in placenta of small- and large-for-gestational-age newborns.The Journal of clinical endocrinology and metabolism · 2010Article
- Estrogen receptor alpha (ERalpha) mediates stimulatory effects of estrogen on aromatase (CYP19) gene expression in human placenta.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Evidence for transcriptional activity in the syncytiotrophoblast of the human placenta.Placenta · 2009Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The hGH cluster contains a single human pituitary growth hormone gene (hGH-N) and four placenta-specific paralogs. Activation of the cluster in both tissues depends on 5' remote regulatory elements. The pituitary-specific locus control elements DNase I-hypersensitive site I (HSI) and HSII, located 14.5 kb 5' of the cluster (position -14.5), establish a continuous domain of histone acetylation that extends to and activates hGH-N in the pituitary gland. In contrast, histone modifications in placental chromatin are restricted to the more 5'-remote HSV-HSIII region (kb -28 to -32) and to the placentally expressed genes in the cluster, with minimal modification between these two regions. These data predict distinct modes of hGH cluster gene activation in the pituitary and placenta. Here we used cell culture models to track structural changes at the hGH locus through placental-gene activation. The data revealed that this process was initiated in primary cytotrophoblasts by histone H3K4 di- and trimethylation and H4 acetylation restricted to HSV and to the individual placental-gene repeat (PGR) units within the cluster. Later stages of transcriptional induction were accompanied by enhancement and extension of these modifications and by robust H3 acetylation at HSV, at HSIII, and throughout the placental-gene regions. These data suggested that elements restricted to HSIII-HSV regions and each individual PGR might be sufficient for activation of the hCS genes. This model was tested by comparing hCS transgene expression in the placentas of mouse embryos carrying a full hGH cluster to that in placentas in which the HSIII-HSV region was directly linked to the individual hCS-A PGR unit. The findings indicate that the HSIII-HSV region and the PGR units, although targeted for initial chromatin structural modifications, are insufficient to activate gene expression and that this process is dependent on additional, as-yet-unidentified chromatin determinants.
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