ArticleMolecular and cellular biology2007
An interaction between two RNA binding proteins, Nab2 and Pub1, links mRNA processing/export and mRNA stability.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA.Molecular and cellular biology · 2016Article
- The Polyadenosine RNA-binding Protein, Zinc Finger Cys3His Protein 14 (ZC3H14), Regulates the Pre-mRNA Processing of a Key ATP Synthase Subunit mRNA.The Journal of biological chemistry · 2016Article
- An Antibody to Detect Alanine-Expanded PABPN1: A New Tool to Study Oculopharyngeal Muscular Dystrophy.Journal of neuromuscular diseases · 2015Article
- Only a subset of the PAB1-mRNP proteome is present in mRNA translation complexes.Protein science : a publication of the Protein Society · 2014Article
- The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay.PLoS genetics · 2013Article
- H2B ubiquitylation controls the formation of export-competent mRNP.Molecular cell · 2012Article
- The mitogen-activated protein kinase Slt2 regulates nuclear retention of non-heat shock mRNAs during heat shock-induced stress.Molecular and cellular biology · 2010Article
- Article
- Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer.Oncogene · 2009Article
- Nuclear transport factor directs localization of protein synthesis during mitosis.Nature cell biology · 2009Article
- Crystallization and preliminary crystallographic analysis of the second RRM of Pub1 from Saccharomyces cerevisiae.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Poly(A) RNA-binding proteins and polyadenosine RNA: new members and novel functions.Wiley interdisciplinary reviews. RNAReview
- Perspectives on the ARE as it turns 25 years old.Wiley interdisciplinary reviews. RNAArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
mRNA stability is modulated by elements in the mRNA transcript and their cognate RNA binding proteins. Poly(U) binding protein 1 (Pub1) is a cytoplasmic Saccharomyces cerevisiae mRNA binding protein that stabilizes transcripts containing AU-rich elements (AREs) or stabilizer elements (STEs). In a yeast two-hybrid screen, we identified nuclear poly(A) binding protein 2 (Nab2) as being a Pub1-interacting protein. Nab2 is an essential nucleocytoplasmic shuttling mRNA binding protein that regulates poly(A) tail length and mRNA export. The interaction between Pub1 and Nab2 was confirmed by copurification and in vitro binding assays. The interaction is mediated by the Nab2 zinc finger domain. Analysis of the functional link between these proteins reveals that Nab2, like Pub1, can modulate the stability of specific mRNA transcripts. The half-life of the RPS16B transcript, an ARE-like sequence-containing Pub1 target, is decreased in both nab2-1 and nab2-67 mutants. In contrast, GCN4, an STE-containing Pub1 target, is not affected. Similar results were obtained for other ARE- and STE-containing Pub1 target transcripts. Further analysis reveals that the ARE-like sequence is necessary for Nab2-mediated transcript stabilization. These results suggest that Nab2 functions together with Pub1 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA turnover in the cytoplasm.
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