ArticleInfection and immunity2007
Functional consequences of sequence variation in bundlin, the enteropathogenic Escherichia coli type IV pilin protein.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Type IV pili ofEcoSal Plus · 2024Review
- High throughput and targeted screens for prepilin peptidase inhibitors do not identify common inhibitors of eukaryotic gamma-secretase.Expert opinion on drug discovery · 2023Article
- BfpI, BfpJ, and BfpK Minor Pilins Are Important for the Function and Biogenesis of Bundle-Forming Pili Expressed by Enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Identification, immunogenicity, and cross-reactivity of type IV pilin and pilin-like proteins from Clostridium difficile.Pathogens and disease · 2014Article
- Type IV pilin proteins: versatile molecular modules.Microbiology and molecular biology reviews : MMBR · 2012Review
- BfpL is essential for type IV bundle-forming pilus biogenesis and interacts with the periplasmic face of BfpC.Microbiology (Reading, England) · 2012Article
- Structure of an essential type IV pilus biogenesis protein provides insights into pilus and type II secretion systems.Journal of molecular biology · 2012Article
- Outer membrane targeting, ultrastructure, and single molecule localization of the enteropathogenic Escherichia coli type IV pilus secretin BfpB.Journal of bacteriology · 2012Article
- The Cpx envelope stress response both facilitates and inhibits elaboration of the enteropathogenic Escherichia coli bundle-forming pilus.Molecular microbiology · 2010Article
- N-acetyllactosamine-induced retraction of bundle-forming pili regulates virulence-associated gene expression in enteropathogenic Escherichia coli.Molecular microbiology · 2010Article
- Interactions of enteropathogenic Escherichia coli with pediatric and adult intestinal biopsy specimens during early adherence.Infection and immunity · 2009Article
- From alpha to beta: identification of amino acids required for the N-acetyllactosamine-specific lectin-like activity of bundlin.Molecular microbiology · 2009Article
- The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine-specific lectin.Cellular microbiology · 2008Article
- Review
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Authors and funding
3 authors.
Funding
Abstract
The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an important virulence factor. We examined the role of divergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactions, and immune responses. We found that the BFP biogenesis machine from an EPEC strain that expresses one bundlin type is capable of assembling all other bundlin types. Furthermore, we found that EPEC strains expressing divergent bundlin types are capable of forming mixed autoaggregates, suggesting that different pilin types can intertwine. However, we found that there was a marked difference between alleles in immunogenicity in both rabbits and mice of a peptide derived from a region of bundlin undergoing apparent diversifying selection. In addition, despite a high degree of cross-reactivity between divergent bundlin proteins, in both mice and rabbits responses appeared to be stronger against the homologous pilin protein than against the heterologous protein. This result was verified using sera from a volunteer study, which demonstrated that the human antibody responses after an initial challenge with live EPEC were stronger against the homologous bundlin protein than against a divergent bundlin protein. However, a repeat challenge induced equivalent responses. These results are consistent with the hypothesis that human immune responses against bundlin exert selective pressure on bfpA sequence divergence.
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