ArticleJournal of clinical microbiology2007
Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed.
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- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Uncommon Salmonella Infantis Variants with Incomplete Antigenic Formula in the Poultry Food Chain, Italy.Emerging infectious diseases · 2024Article
- bettercallsal: better calling ofFrontiers in microbiology · 2023Article
- Genotypic virulence profiles and associations in Salmonella isolated from meat samples in wet markets and abattoirs of Metro Manila, Philippines.BMC microbiology · 2022Article
- Changes in the genomes and methylomes of threeFrontiers in microbiology · 2022Article
- Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.PloS one · 2021Article
- Development of a Genoserotyping Method forMicroorganisms · 2020Article
- Comparison of xMAPFrontiers in cellular and infection microbiology · 2020Article
- SeqSero2: Rapid and ImprovedApplied and environmental microbiology · 2019Article
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- Diversity and Dynamics ofFrontiers in microbiology · 2019Article
- CharacterizingFrontiers in veterinary science · 2019Article
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- Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples.Applied and environmental microbiology · 2017Article
- xMAP Technology: Applications in Detection of Pathogens.Frontiers in microbiology · 2017Review
- Evaluation of a multiplex PCR method to serotypeMethodsX · 2017Article
- Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the development and evaluation of a Salmonella O-group-specific Bio-Plex assay to detect the six most common serogroups in the United States (B, C(1), C(2), D, E, and O13) plus serotype Paratyphi A. The assay is based on rfb gene targets directly involved in O-antigen biosynthesis; it can be completed 45 min post-PCR amplification. The assay correctly and specifically identified 362 of 384 (94.3%) isolates tested in comparison to traditional serotyping. Seventeen isolates (4.4%) produced results consistent with what is known about the molecular basis for serotypes but different from the results of traditional serotyping, and five isolates (1.3%) generated false-negative results. Molecular determination of the serogroup for rough isolates was consistent with a common serotype in most instances, indicating that this approach has the potential to provide O-group information for isolates that do not express an O antigen. We also report the sequence of the O-antigen-encoding rfb gene cluster from Salmonella enterica serotype Poona (serogroup O13). Compared with other, previously characterized rfb regions, the O13 rfb gene cluster was most closely related to Escherichia coli O127 and O86. The O-group Bio-Plex assay described here provides an easy-to-use, high-throughput system for rapid detection of common Salmonella serogroups.
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