ArticleMolecular biology of the cell2007
ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance.Journal of proteome research · 2021Article
- The Capture of a Disabled Proteasome Identifies Erg25 as a Substrate for Endoplasmic Reticulum Associated Degradation.Molecular & cellular proteomics : MCP · 2020Article
- Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.The Journal of biological chemistry · 2017Article
- Subcellular Fractionation Analysis of the Extraction of Ubiquitinated Polytopic Membrane Substrate during ER-Associated Degradation.PloS one · 2016Article
- iRhom1 regulates proteasome activity via PAC1/2 under ER stress.Scientific reports · 2015Article
- Yeast importin-α (Srp1) performs distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus.The Journal of biological chemistry · 2014Article
- The Cdc48-Vms1 complex maintains 26S proteasome architecture.The Biochemical journal · 2014Article
- Assembly of the 20S proteasome.Biochimica et biophysica acta · 2014Review
- The unfolded protein response in fatty liver disease.Seminars in liver disease · 2013Review
- The Lhs1/GRP170 chaperones facilitate the endoplasmic reticulum-associated degradation of the epithelial sodium channel.The Journal of biological chemistry · 2013Article
- The delicate balance between secreted protein folding and endoplasmic reticulum-associated degradation in human physiology.Physiological reviews · 2012Review
- Proteostasis: a new therapeutic paradigm for pulmonary disease.Proceedings of the American Thoracic Society · 2011Article
- Mechanisms underlying the cellular clearance of antitrypsin Z: lessons from yeast expression systems.Proceedings of the American Thoracic Society · 2010Article
- Molecular chaperones and substrate ubiquitination control the efficiency of endoplasmic reticulum-associated degradation.Diabetes, obesity & metabolism · 2010Review
- Endoplasmic reticulum-associated degradation (ERAD) and autophagy cooperate to degrade polymerogenic mutant serpins.The Journal of biological chemistry · 2009Article
- A novel hybrid yeast-human network analysis reveals an essential role for FNBP1L in antibacterial autophagy.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Molecular mechanisms of proteasome assembly.Nature reviews. Molecular cell biology · 2009Review
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Antitrypsin deficiency is a primary cause of juvenile liver disease, and it arises from expression of the "Z" variant of the alpha-1 protease inhibitor (A1Pi). Whereas A1Pi is secreted from the liver, A1PiZ is retrotranslocated from the endoplasmic reticulum (ER) and degraded by the proteasome, an event that may offset liver damage. To better define the mechanism of A1PiZ degradation, a yeast expression system was developed previously, and a gene, ADD66, was identified that facilitates A1PiZ turnover. We report here that ADD66 encodes an approximately 30-kDa soluble, cytosolic protein and that the chymotrypsin-like activity of the proteasome is reduced in add66Delta mutants. This reduction in activity may arise from the accumulation of 20S proteasome assembly intermediates or from qualitative differences in assembled proteasomes. Add66p also seems to be a proteasome substrate. Consistent with its role in ER-associated degradation (ERAD), synthetic interactions are observed between the genes encoding Add66p and Ire1p, a transducer of the unfolded protein response, and yeast deleted for both ADD66 and/or IRE1 accumulate polyubiquitinated proteins. These data identify Add66p as a proteasome assembly chaperone (PAC), and they provide the first link between PAC activity and ERAD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.