ArticleNature biotechnology2007
Molecular breeding of polymerases for amplification of ancient DNA.
Article in Nature biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 121 citations in OpenAlex.
- Rapid evolution of a highly efficient RNA polymerase by homologous recombination.Nature chemical biology · 2026Article
- Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences.Nature communications · 2026Article
- Nucleic acid joining enzymes: biological functions and synthetic applications beyond DNA.The Biochemical journal · 2025Review
- DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.International journal of molecular sciences · 2023Review
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma.RSC chemical biology · 2022Review
- Nucleobase-modified nucleosides and nucleotides: Applications in biochemistry, synthetic biology, and drug discovery.Frontiers in chemistry · 2022Review
- Characterization of FFPE-induced bacterial DNA damage and development of a repair method.Biology methods & protocols · 2020Article
- Engineering Polymerases for New Functions.Trends in biotechnology · 2019Review
- Synthetic evolution.Nature biotechnology · 2019Review
- Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR.Nucleic acids research · 2019Article
- Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library.Scientific reports · 2019Article
- Oxidatively-induced DNA damage and base excision repair in euthymic patients with bipolar disorder.DNA repair · 2018Article
- Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.Nature protocols · 2017Review
- Exploring the Chemistry of Genetic Information Storage and Propagation through Polymerase Engineering.Accounts of chemical research · 2017Review
- Human evolution: a tale from ancient genomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- A polymerase engineered for bisulfite sequencing.Nucleic acids research · 2015Article
- DNA polymerase from temperate phage Bam35 is endowed with processive polymerization and abasic sites translesion synthesis capacity.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Methods for the directed evolution of proteins.Nature reviews. Genetics · 2015Review
- Reconstructing ancient genomes and epigenomes.Nature reviews. Genetics · 2015Review
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9 authors at 6 institutions in 4 countries.
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Abstract
In the absence of repair, lesions accumulate in DNA. Thus, DNA persisting in specimens of paleontological, archaeological or forensic interest is inevitably damaged. We describe a strategy for the recovery of genetic information from damaged DNA. By molecular breeding of polymerase genes from the genus Thermus (Taq (Thermus aquaticus), Tth (Thermus thermophilus) and Tfl (Thermus flavus)) and compartmentalized self-replication selection, we have evolved polymerases that can extend single, double and even quadruple mismatches, process non-canonical primer-template duplexes and bypass lesions found in ancient DNA, such as hydantoins and abasic sites. Applied to the PCR amplification of 47,000-60,000-year-old cave bear DNA, these outperformed Taq DNA polymerase by up to 150% and yielded amplification products at sample dilutions at which Taq did not. Our results demonstrate that engineered polymerases can expand the recovery of genetic information from Pleistocene specimens and may benefit genetic analysis in paleontology, archeology and forensic medicine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.