ArticleJournal of bacteriology2007
VirB1* promotes T-pilus formation in the vir-Type IV secretion system of Agrobacterium tumefaciens.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- MtrR Regulates a Major Lytic Transglycosylase (Microorganisms · 2026Article
- Type IV secretion systems: reconciling diversity through a unified nomenclature.FEMS microbiology reviews · 2026Review
- Morphogenetic penicillin-binding proteins control virulence-associated type III secretion systems inInfection and immunity · 2025Article
- Beyond survival to domination:Frontiers in microbiology · 2025Review
- Article
- The Effect of Outer Space and Other Environmental Cues on Bacterial Conjugation.Microbiology spectrum · 2023Article
- Peptidoglycan deacetylation controls type IV secretion and the intracellular survival of the bacterial pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Comparative Genomic Analysis ofFrontiers in microbiology · 2022Article
- The VirB System Plays a Crucial Role inInternational journal of molecular sciences · 2021Review
- Comparative genome analysis ofMicrobial genomics · 2019Article
- Two pKM101-encoded proteins, the pilus-tip protein TraC and Pep, assemble on the Escherichia coli cell surface as adhesins required for efficient conjugative DNA transfer.Molecular microbiology · 2019Article
- Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens.Microbiology and molecular biology reviews : MMBR · 2018Review
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Article
- The Mosaic Type IV Secretion Systems.EcoSal Plus · 2016Review
- PopZ identifies the new pole, and PodJ identifies the old pole during polar growth in Agrobacterium tumefaciens.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Recent advances in the structural and molecular biology of type IV secretion systems.Current opinion in structural biology · 2014Review
- The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.Journal of bacteriology · 2014Article
- Chromobacterium violaceum: important insights for virulence and biotechnological potential by exoproteomic studies.Current microbiology · 2013Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the outside of the bacterial cell, suggesting an additional function. We show that both nopaline and octopine strains produce abundant amounts of VirB1* and perform detailed studies on nopaline VirB1*. Both domains are required for wild-type virulence. We show here that the nopaline type VirB1* is essential for the formation of the T pilus, a subassembly of the vir-T4SS composed of processed and cyclized VirB2 (major subunit) and VirB5 (minor subunit). A nopaline virB1 deletion strain does not produce T pili. Complementation with full-length VirB1 or C-terminal VirB1*, but not the N-terminal lytic transglycosylase domain, restores T pili containing VirB2 and VirB5. T-pilus preparations also contain extracellular VirB1*. Protein-protein interactions between VirB1* and VirB2 and VirB5 were detected in the yeast two-hybrid assay. We propose that VirB1 is a bifunctional protein required for virT4SS assembly. The N-terminal lytic transglycosylase domain provides localized lysis of the peptidoglycan cell wall to allow insertion of the T4SS. The C-terminal VirB1* promotes T-pilus assembly through protein-protein interactions with T-pilus subunits.
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