ArticleBiophysical journal2007
Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 104 citations in OpenAlex.
- Single-Cell Analysis with Spatiotemporal Control of Local pH.ACS measurement science au · 2025Article
- Precision Delivery Using Nanopipette for Single-cell Studies.Dian hua xue · 2024Article
- Article
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Rational design of DNA nanostructures for single molecule biosensing.Nature communications · 2020Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Nanopipette/Nanorod-Combined Quartz Tuning Fork⁻Atomic Force Microscope.Sensors (Basel, Switzerland) · 2019Article
- MoNa - A Cost-Efficient, Portable System for the Nanoinjection of Living Cells.Scientific reports · 2019Article
- Review
- Multifunctional scanning ion conductance microscopy.Proceedings. Mathematical, physical, and engineering sciences · 2017Review
- Survival rate of eukaryotic cells following electrophoretic nanoinjection.Scientific reports · 2017Article
- From single molecules to life: microscopy at the nanoscale.Analytical and bioanalytical chemistry · 2016Review
- Resistive-Pulse Measurements with Nanopipettes: Detection of Vascular Endothelial Growth Factor C (VEGF-C) Using Antibody-Decorated Nanoparticles.Analytical chemistry · 2015Article
- Barriers to the free diffusion of proteins and lipids in the plasma membrane.The Journal of cell biology · 2015Review
- Hierarchical hydrodynamic flow confinement: efficient use and retrieval of chemicals for microscale chemistry on surfaces.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Nanopipette exploring nanoworld.Nano convergence · 2014Review
- Imaging the cell surface and its organization down to the level of single molecules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- A quantitative comparison of single-dye tracking analysis tools using Monte Carlo simulations.PloS one · 2013Article
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
- A fractal model for nuclear organization: current evidence and biological implications.Nucleic acids research · 2012Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new method, using a nanopipette, for controlled voltage-driven delivery of individual fluorescently labeled probe molecules to the plasma membrane which we used for single-molecule fluorescence tracking (SMT). The advantages of the method are 1), application of the probe to predefined regions on the membrane; 2), release of only one or a few molecules onto the cell surface; 3), when combined with total internal reflection fluorescence microscopy, very low background due to unbound molecules; and 4), the ability to first optimize the experiment and then repeat it on the same cell. We validated the method by performing an SMT study of the diffusion of individual membrane glycoproteins labeled with Atto 647-wheat germ agglutin in different surface domains of boar spermatozoa. We found little deviation from Brownian diffusion with a mean diffusion coefficient of 0.79 +/- 0.04 microm(2)/s in the acrosomal region and 0.10 +/- 0.02 microm(2)/s in the postacrosomal region; this difference probably reflects different membrane structures. We also showed that we can analyze diffusional properties of different subregions of the cell membrane and probe for the presence of diffusion barriers. It should be straightforward to extend this new method to other probes and cells, and it can be used as a new tool to investigate the cell membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.