ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2007
A novel three-dimensional in vitro system to study trophoblast-endothelium cell interactions.
Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 77 citations in OpenAlex.
- Microglia alter sex-specific cerebellar myelination following placental hormone loss.Nature communications · 2025Article
- Evaluation of optimal trophoblastic models to mimic uterine vessel remodeling in human pregnancy†.Biology of reproduction · 2025Article
- Interleukin-1β Stimulates Matrix Metalloproteinase 10 Secretion: A Possible Mechanism in Trophoblast-Dependent Spiral Artery Remodeling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Identification of the role of DAB2 and CXCL8 in uterine spiral artery remodeling in early-onset preeclampsia.Cellular and molecular life sciences : CMLS · 2024Article
- Fibronectin mediates activin A-promoted human trophoblast migration and acquisition of endothelial-like phenotype.Cell communication and signaling : CCS · 2024Article
- Editorial: Advances and perspectives in neuroplacentology.Frontiers in endocrinology · 2023Article
- Placental trophoblast-specific overexpression of chemerin induces preeclampsia-like symptoms.Clinical science (London, England : 1979) · 2022Article
- Article
- Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells.International journal of molecular sciences · 2020Article
- Pro-angiogenic effects of pregnancy-specific glycoproteins in endothelial and extravillous trophoblast cells.Reproduction (Cambridge, England) · 2020Article
- Insight Into TLR4-Mediated Immunomodulation in Normal Pregnancy and Related Disorders.Frontiers in immunology · 2020Review
- Pathogenic Autoreactive T and B Cells Cross-React with Mimotopes Expressed by a Common Human Gut Commensal to Trigger Autoimmunity.Cell host & microbe · 2019Article
- Low molecular weight heparin and aspirin exacerbate human endometrial endothelial cell responses to antiphospholipid antibodies.American journal of reproductive immunology (New York, N.Y. : 1989) · 2018Article
- dNK cells facilitate the interaction between trophoblastic and endothelial cells via VEGF-C and HGF.Immunology and cell biology · 2017Article
- Epithelial membrane protein 2 (EMP2) deficiency alters placental angiogenesis, mimicking features of human placental insufficiency.The Journal of pathology · 2017Article
- Establishment of the Human Uteroplacental Circulation: A Historical Perspective.Reproductive sciences (Thousand Oaks, Calif.) · 2017Review
- Interferon-α and angiogenic dysregulation in pregnant lupus patients who develop preeclampsia.Arthritis & rheumatology (Hoboken, N.J.) · 2015Article
- The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.PloS one · 2014Article
- Human endometrial endothelial cells generate distinct inflammatory and antiviral responses to the TLR3 agonist, Poly(I:C) and the TLR8 agonist, viral ssRNA.American journal of reproductive immunology (New York, N.Y. : 1989) · 2013Article
- A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation.Fertility and sterility · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
introductionPregnancy complications have been linked to improper trophoblast migration and failure of spiral artery transformation. Endothelial cells play an essential role in directing trophoblast migration and transformation, although by an unknown mechanism. We describe a novel in vitro model to evaluate endothelial-trophoblast interaction and signaling in a three-dimensional system. METHOD OF STUDY: Immortalized human endometrial endothelial cell line and first trimester trophoblast cells were co-cultured. Endothelial transformation into vessel-like structures occurred in Matrigel(TM) OpenLab Image Analysis software was used to monitor labeled trophoblast migration and endothelium transformation. Cytokine/chemokine production was determined using Multiplex.
resultsTrophoblast migrates toward endothelial cells in Matrigel, aligns on top of the endothelium within 4-8 hr and achieves complete replacement of the endothelium by 72-96 hr. Lipopolysaccharide treatment damages the endothelium and disrupts endothelium-trophoblast interaction.
conclusionWe report a novel three-dimensional in vitro and in vivo system of trophoblast-endothelium cell interaction. Significant changes in endothelial cells' phenotype are observed upon differentiation in Matrigel. These changes may be necessary for endothelium to direct trophoblast migration and transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.