ArticlePLoS pathogens2007
The OmpA-like protein Loa22 is essential for leptospiral virulence.
Article in PLoS pathogens, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers, 1 of them a synthesis that pooled it.
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Who cites it
118 citing papers in PubMed, 1 synthesis or guideline pooled it, 253 citations in OpenAlex.
- Trends and advances inFrontiers in microbiology · 2024Pooled it
- A recombinant Loa22-based latex agglutination test and indirect ELISA for the sero-detection of leptospirosis in dogs and cattle.Archives of microbiology · 2026Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transposon Sequencing in Leptospira interrogans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Comparative Genome Analysis of 25 Sri LankanEvolutionary bioinformatics online · 2025Article
- Identification of equine mares as reservoir hosts for pathogenic species ofFrontiers in veterinary science · 2024Article
- Immunological characterization of recombinant outer membrane Loa22 protein of local pathogenicVeterinary research forum : an international quarterly journal · 2024Article
- Molecular Characterization of Gene Encoding Outer Membrane ProteinJournal of tropical medicine · 2024Article
- The leptospiral OmpA-like protein (Loa22) is a surface-exposed antigen that elicits bactericidal antibody against heterologousVaccine: X · 2023Article
- Improvement the expression and purification of Loa22: a lipoprotein with OmpA domain from pathogenicIranian journal of microbiology · 2023Article
- Evaluation ofFrontiers in microbiology · 2023Article
- Interaction of Liberibacter Solanacearum with Host Psyllid Vitellogenin and Its Association with Autophagy.Microbiology spectrum · 2022Article
- Bovine leptospirosis: effects on reproduction and an approach to research in Colombia.Tropical animal health and production · 2022Review
- Insight into the Structure, Functions, and Dynamics of theMembranes · 2022Review
- Host and Species-Specificities of Pattern Recognition Receptors Upon Infection WithFrontiers in cellular and infection microbiology · 2022Review
- A Novel Breakthrough inFrontiers in microbiology · 2022Article
- Screening of Surface-Exposed Lipoproteins ofFrontiers in microbiology · 2022Article
- Transposable Prophages in Leptospira: An Ancient, Now Diverse, Group Predominant in Causative Agents of Weil's Disease.International journal of molecular sciences · 2021Article
- Spatio-temporal distribution of bovine leptospirosis in Tamil Nadu and a risk factor analysis.Veterinarni medicina · 2021Article
- Surface proteomics and label-free quantification of Leptospira interrogans serovar Pomona.PLoS neglected tropical diseases · 2021Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.