ArticleRNA (New York, N.Y.)2007
Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Article
- Alternative Splicing and Transcription Elongation in Plants.Frontiers in plant science · 2019Review
- Splicing Factor RBM20 Regulates Transcriptional Network of Titin Associated and Calcium Handling Genes in The Heart.International journal of biological sciences · 2018Article
- Splicing of designer exons informs a biophysical model for exon definition.RNA (New York, N.Y.) · 2015Article
- Pre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate.Genes & development · 2014Article
- Reflections on the history of pre-mRNA processing and highlights of current knowledge: a unified picture.RNA (New York, N.Y.) · 2013Review
- Alternative splicing: a pivotal step between eukaryotic transcription and translation.Nature reviews. Molecular cell biology · 2013Review
- In vitro transcription and capping of Gaussia luciferase mRNA followed by HeLa cell transfection.Journal of visualized experiments : JoVE · 2012Article
- Review
- RNA Polymerase II Elongation at the Crossroads of Transcription and Alternative Splicing.Genetics research international · 2011Article
- A model in vitro system for co-transcriptional splicing.Nucleic acids research · 2010Article
- TLS inhibits RNA polymerase III transcription.Molecular and cellular biology · 2010Article
- "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.Molecular cell · 2009Review
- Cotranscriptional splicing potentiates the mRNA production from a subset of estradiol-stimulated genes.Molecular and cellular biology · 2008Article
- Functional integration of transcriptional and RNA processing machineries.Current opinion in cell biology · 2008Review
- Functional coupling of last-intron splicing and 3'-end processing to transcription in vitro: the poly(A) signal couples to splicing before committing to cleavage.Molecular and cellular biology · 2008Article
- Light in the transcription landscape: chromatin, RNA polymerase II and splicing throughoutTranscriptionReview
- Pre-mRNA splicing during transcription in the mammalian system.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The concept of a tight integration of transcription and splicing of mRNA precursors has been supported with increasing evidence in recent years. However, the mechanism and functional consequences of this integration remain largely unknown. We have examined how these processes impact upon one another when they occur together in HeLa nuclear extract. While both processes do in fact occur in parallel reactions in the extracts, we found no evidence that one process affects the other, under a variety of conditions tested. For example, neither the kinetics nor efficiency of splicing is significantly enhanced by de novo RNA polymerase II-mediated transcription, relative to that of presynthesized RNA added exogenously to the extract. Our results indicate that the act of transcription by RNA polymerase II in vitro is not sufficient to enhance splicing of the newly made RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.