ArticleNucleic acids research1991
Transcription factor binding and spacing constraints in the human beta-actin proximal promoter.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- Multilayered regulation of cytoskeletal protein abundance: autoregulatory mechanisms of actin and tubulin.Experimental & molecular medicine · 2026Review
- Cloning, characterization, and transcriptional activity of β-actin promoter of African catfish (Clarias gariepinus).Molecular biology reports · 2021Article
- Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells.Journal of biological engineering · 2019Article
- The Hap Complex in Yeasts: Structure, Assembly Mode, and Gene Regulation.Frontiers in microbiology · 2019Review
- Upregulation of the actin cytoskeleton via myocardin leads to increased expression of type 1 collagen.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Article
- Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription.Biochimica et biophysica acta · 2016Article
- Characterization of the octamer, a cis-regulatory element that modulates excretory cell gene-expression in Caenorhabditis elegans.BMC molecular biology · 2010Article
- A survey of 178 NF-Y binding CCAAT boxes.Nucleic acids research · 1998Review
- Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.Molecular and cellular biology · 1997Article
- Characterization of the estrogen receptor transfected MCF10A breast cell line 139B6.Breast cancer research and treatment · 1996Article
- CCAAT-box binding protein NF-Y (CBF, CP1) recognizes the minor groove and distorts DNA.Nucleic acids research · 1995Article
- EFIA/YB-1 is a component of cardiac HF-1A binding activity and positively regulates transcription of the myosin light-chain 2v gene.Molecular and cellular biology · 1995Article
- Negative regulation of the vascular smooth muscle alpha-actin gene in fibroblasts and myoblasts: disruption of enhancer function by sequence-specific single-stranded-DNA-binding proteins.Molecular and cellular biology · 1995Article
- CCAAT box binding protein NF-Y facilitates in vivo recruitment of upstream DNA binding transcription factors.The EMBO journal · 1994Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional activity of, and the protein-DNA interactions that occur within, the proximal region of the human beta-actin promoter. Efficient beta-actin promoter activity in transfected human HeLa cells requires only 114bp of 5' flanking sequences. Two of the cis-actin regulatory elements within this region of the beta-actin promoter, the CCAAT box and proximal CCArGG box, are specific in vitro binding sites for the transcription factors, nuclear factor Y (NF-Y) and serum response factor (p67SRF), respectively. These two elements are required together to stimulate in vivo transcription from the homologous as well as a heterologous promoter. Finally, a particular spatial alignment between the CCAAT box and proximal CCArGG box is required for trans-activation in vivo. The above provides strong evidence for a functional interaction between NF-Y and p67SRF when bound to their respective binding sites in the beta-actin promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.