ArticleNucleic acids research1991
Presence of regulatory sequences within intron 2 of the mouse thymidine kinase gene.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- Interaction of Sp1 with the growth- and cell cycle-regulated transcription factor E2F.Molecular and cellular biology · 1996Article
- Expression of carbonic anhydrase II (CA II) promoter-reporter fusion genes in multiple tissues of transgenic mice does not replicate normal patterns of expression indicating complexity of CA II regulation in vivo.Biochemical genetics · 1995Article
- Interaction of several related GC-box- and GT-box-binding proteins with the intronic enhancer is required for differential expression of the gb110 gene in embryonal carcinoma cells.Molecular and cellular biology · 1994Article
- Presence of a regulatory element within the first intron of the human platelet-derived growth factor-A chain gene.Japanese journal of cancer research : Gann · 1993Article
- A binding site for transcription factor E2F is a target for trans activation of murine thymidine kinase by polyomavirus large T antigen and plays an important role in growth regulation of the gene.Journal of virology · 1993Article
- An S-phase specific release from a transcriptional block regulates the expression of mouse ribonucleotide reductase R2 subunit.The EMBO journal · 1992Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1992Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and two new protein binding regions, one at the promoter proximal end of intron 2, the other one close to the border to exon 3 were found. Oligonucleotides were synthesized comprising the two new binding sites and were shown in gel mobility shift experiments to be capable of forming specific complexes with nuclear proteins. These proteins are present in growing as well as in quiescent cells suggesting that the sites described here do not contribute to growth regulation of TK expression. That they might play a role in upregulation of TK expression is, however, indicated by the results of CAT assays in which inclusion of downstream sequences of the TK gene containing parts or all of intron 2 were found to positively modulate the activity of the TK promoter.
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