ArticleRNA (New York, N.Y.)2007
FRET-detectable interactions between the ARE binding proteins, HuR and p37AUF1.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 72 citations in OpenAlex.
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- Phosphorylation at the disordered N-end makes HuR accumulate and dimerize in the cytoplasm.Nucleic acids research · 2024Article
- Mapping RNA-protein interactions with subcellular resolution using colocalization CLIP.RNA (New York, N.Y.) · 2024Article
- Hu Antigen R (HuR) Protein Structure, Function and Regulation in Hepatobiliary Tumors.Cancers · 2022Review
- The versatile role of HuR in Glioblastoma and its potential as a therapeutic target for a multi-pronged attack.Advanced drug delivery reviews · 2022Review
- Triciribine Engages ZFP36L1 and HuR to Stabilize LDLR mRNA.Molecules (Basel, Switzerland) · 2020Article
- Effect of Inhibiting p38 on HuR Involving in β-AChR Post-transcriptional Mechanisms in Denervated Skeletal Muscle.Cellular and molecular neurobiology · 2019Article
- Hu antigen R (HuR) multimerization contributes to glioma disease progression.The Journal of biological chemistry · 2017Article
- AUF1 regulation of coding and noncoding RNA.Wiley interdisciplinary reviews. RNA · 2017Review
- RNA-binding proteins regulate cell respiration and coenzyme Q biosynthesis by post-transcriptional regulation of COQ7.RNA biology · 2016Article
- Different motif requirements for the localization zipcode element of β-actin mRNA binding by HuD and ZBP1.Nucleic acids research · 2015Article
- Immunological visibility: posttranscriptional regulation of human NKG2D ligands by the EGF receptor pathway.Science translational medicine · 2014Article
- The C-terminal RNA binding motif of HuR is a multi-functional domain leading to HuR oligomerization and binding to U-rich RNA targets.RNA biology · 2014Article
- Reduction of AUF1-mediated follistatin mRNA decay during glucose starvation protects cells from apoptosis.Nucleic acids research · 2014Article
- Cellular mRNA decay protein AUF1 negatively regulates enterovirus and human rhinovirus infections.Journal of virology · 2013Article
- Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction.RNA (New York, N.Y.) · 2013Review
- Post-transcriptional regulatory networks in immunity.Immunological reviews · 2013Review
- All three RNA recognition motifs and the hinge region of HuC play distinct roles in the regulation of alternative splicing.Nucleic acids research · 2013Article
- HuR-hnRNP interactions and the effect of cellular stress.Molecular and cellular biochemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A number of highly regulated gene classes are regulated post-transcriptionally at the level of mRNA stability. A central feature in these mRNAs is the presence of A+U-rich elements (ARE) within their 3' UTRs. Two ARE binding proteins, HuR and AUF1, are associated with mRNA stabilization and destabilization, respectively. Previous studies have demonstrated homomultimerization of each protein and the capacity to bind simultaneous or competitively to a single ARE. To investigate this possibility further, cell biological and biophysical approaches were undertaken. Protein-protein interaction was monitored by fluorescence resonance energy transfer (FRET) and by immunocytochemistry in live and fixed cells using fluorescently labeled CFP/YFP fusion proteins of HuR and p37AUF1. Strong nuclear FRET between HuR/HuR and AUF1/AUF1 homodimers as well as HuR/AUF1 heterodimers was observed. Treatment with the MAP kinase activator, anisomycin, which commonly stabilizes ARE-containing mRNAs, caused rapid nuclear to cytoplasmic shuttling of HuR. AUF1 also underwent shuttling, but on a longer time scale. After shuttling, HuR/HuR, AUF1/AUF1, and HuR/AUF1, FRET was also observed in the cytoplasm. In further studies, arsenite rapidly induced the formation of stress granules containing HuR and TIA-1 but not AUF1. The current studies demonstrate that two mRNA binding proteins, HuR and AUF1, are colocalized and are capable of functional interaction in both the nucleus and cytoplasm. FRET-based detection of AUF1/HuR interaction may serve as a basis of opening up new dimensions in delineating the functional interaction of mRNA binding proteins with RNA turnover.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.