ArticleJournal of clinical microbiology2007
New method for simultaneous species-specific identification of equine strongyles (nematoda, strongylida) by reverse line blot hybridization.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Intestinal Strongyle Genera in Different Typology of Donkey Farms in Tuscany, Central Italy.Veterinary sciences · 2020Article
- Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients.Animals : an open access journal from MDPI · 2020Article
- The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins.International journal for parasitology · 2018Article
- Cyathostomine egg reappearance period following ivermectin treatment in a cohort of UK Thoroughbreds.Parasites & vectors · 2018Article
- Nematode Species Identification-Current Status, Challenges and Future Perspectives for Cyathostomins.Frontiers in cellular and infection microbiology · 2017Article
- Efficacy of selected anthelmintic drugs against cyathostomins in horses in the federal state of Brandenburg, Germany.Parasitology research · 2015Article
- Comparison of internal transcribed spacers and intergenic spacer regions of five common Iranian sheep bursate nematodes.Iranian journal of parasitology · 2014Article
- Article
- Fecundity of various species of strongylids (Nematoda: Strongylidae)--parasites of domestic horses.Parasitology research · 2012Article
- The Strongylidae belonging to Strongylus genus in horses from southeastern Poland.Parasitology research · 2012Review
- Observations on development of natural infection and species composition of small strongyles in young equids in Kentucky.Parasitology research · 2011Article
- Three recently recognized species of cyathostomes (Nematoda: Strongylidae) in equids in Kentucky.Parasitology research · 2011Article
- Morphology and diagnosis of the fourth-stage larva of Coronocyclus labratus (Looss, 1900) (Nematoda: Strongyloidea) parasitising equids.Systematic parasitology · 2010Article
- Equine cyathostomins: a review of biology, clinical significance and therapy.Parasites & vectors · 2009Article
- Restrictions of anthelmintic usage: perspectives and potential consequences.Parasites & vectors · 2009Article
- Biotechnological advances in the diagnosis of little-known parasitoses of pets.Parasitology research · 2009Review
- Morphology and diagnosis of some fourth-stage larvae of cyathostomines (Nematoda: Strongyloidea) in donkeys Equus asinus L. from Ethiopia.Systematic parasitology · 2009Article
- Preliminary molecular identification of drug resistant cyathostomes in Italy.Veterinary research communications · 2008Article
- First report of multiple drug resistance in trichostrongyles affecting sheep under field conditions in Italy.Parasitology research · 2007Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and simultaneously discriminated from each other and from three large strongyles (Strongylus edentatus, Strongylus equinus, and Strongylus vulgaris). This assay will enable the accurate and rapid identification of equine cyathostomins irrespective of their life cycle stage, opening important avenues for a better understanding of their biology and epidemiology and of the pathogenesis of cyathostomin-associated disease. In particular, this RLB method promises to be a powerful diagnostic tool to determine the roles of individual species in the pathogenesis of mixed infections and to elucidate some aspects of cyathostominosis. Also, it could represent a basic step toward the development of a rapid and simple molecular test for the early detection of drug-resistant genotypes of horse strongyle species.
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