ArticleJournal of virology2007
Arterivirus subgenomic mRNA synthesis and virion biogenesis depend on the multifunctional nsp1 autoprotease.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
- Discovery of a [4Fe-4S] cluster in the PRRSV Nsp1α leader protease reveals host-virus interplay in its downstream functions.Science advances · 2026Article
- Discovery of a [4Fe-4S] cluster in the PRRSV Nsp1α leader protease reveals host-virus interplay in its downstream functions.bioRxiv : the preprint server for biology · 2026Article
- The dimeric conformation of PRRSV nsp1α is important for its ability to regulate viral RNA synthesis.Veterinary research · 2025Article
- S146 and M148 within the mature chain domain of PSMB4 are crucial for degrading PRRSV nsp1α.Cellular and molecular life sciences : CMLS · 2025Article
- Classification, replication, and transcription ofFrontiers in microbiology · 2023Review
- Structures and functions of coronavirus replication-transcription complexes and their relevance for SARS-CoV-2 drug design.Nature reviews. Molecular cell biology · 2022Review
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): Codon Usage and Replicative Fitness.Global medical genetics · 2020Article
- The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis.Emerging microbes & infections · 2019Article
- Article
- The viral innate immune antagonism and an alternative vaccine design for PRRS virus.Veterinary microbiology · 2017Review
- Domain Organization and Evolution of the Highly Divergent 5' Coding Region of Genomes of Arteriviruses, Including the Novel Possum Nidovirus.Journal of virology · 2017Article
- Targeting Swine Leukocyte Antigen Class I Molecules for Proteasomal Degradation by the nsp1α Replicase Protein of the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Strain JXwn06.Journal of virology · 2016Article
- PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity.Virology · 2015Review
- Re-emergence of a genetic outlier strain of equine arteritis virus: Impact on phylogeny.Virus research · 2015Article
- Modulation of innate immune signaling by nonstructural protein 1 (nsp1) in the family Arteriviridae.Virus research · 2014Review
- Engineering the PRRS virus genome: updates and perspectives.Veterinary microbiology · 2014Review
- Experiences with infectious cDNA clones of equine arteritis virus: lessons learned and insights gained.Virology · 2014Review
- Transactivation of programmed ribosomal frameshifting by a viral protein.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Overview: Replication of porcine reproductive and respiratory syndrome virus.Journal of microbiology (Seoul, Korea) · 2013Review
- Equine arteritis virus.Veterinary microbiology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many groups of plus-stranded RNA viruses produce additional, subgenomic mRNAs to regulate the expression of part of their genome. Arteriviruses and coronaviruses (order Nidovirales) are unique among plus-stranded RNA viruses for using a mechanism of discontinuous RNA synthesis to produce a nested set of 5'- and 3'-coterminal subgenomic mRNAs, which serve to express the viral structural protein genes. The discontinuous step presumably occurs during minus-strand synthesis and joins noncontiguous sequences copied from the 3'- and 5'-proximal domains of the genomic template. Nidovirus genome amplification ("replication") and subgenomic mRNA synthesis ("transcription") are driven by 13 to 16 nonstructural proteins (nsp's), generated by autocatalytic processing of two large "replicase" polyproteins. Previously, using a replicon system, the N-terminal nsp1 replicase subunit of the arterivirus equine arteritis virus (EAV) was found to be dispensable for replication but crucial for transcription. Using reverse genetics, we have now addressed the role of nsp1 against the background of the complete EAV life cycle. Mutagenesis revealed that nsp1 is in fact a multifunctional regulatory protein. Its papain-like autoprotease domain releases nsp1 from the replicase polyproteins, a cleavage essential for viral RNA synthesis. Several mutations in the putative N-terminal zinc finger domain of nsp1 selectively abolished transcription, while replication was either not affected or even increased. Other nsp1 mutations did not significantly affect either replication or transcription but still dramatically reduced the production of infectious progeny. Thus, nsp1 is involved in at least three consecutive key processes in the EAV life cycle: replicase polyprotein processing, transcription, and virion biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.