ArticleJournal of virology2007
PrPc does not mediate internalization of PrPSc but is required at an early stage for de novo prion infection of Rov cells.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Cell biology of prion strains in vivo and in vitro.Cell and tissue research · 2023Review
- An astrocyte cell line that differentially propagates murine prions.The Journal of biological chemistry · 2020Article
- Prion strains depend on different endocytic routes for productive infection.Scientific reports · 2017Article
- Modulation of Glycosaminoglycans Affects PrPSc Metabolism but Does Not Block PrPSc Uptake.Journal of virology · 2015Article
- Generation of a persistently infected MDBK cell line with natural bovine spongiform encephalopathy (BSE).PloS one · 2015Article
- Uptake and degradation of protease-sensitive and -resistant forms of abnormal human prion protein aggregates by human astrocytes.The American journal of pathology · 2014Article
- Article
- A specific population of abnormal prion protein aggregates is preferentially taken up by cells and disaggregated in a strain-dependent manner.Journal of virology · 2013Article
- Cellular aspects of prion replication in vitro.Viruses · 2013Review
- Prions Ex Vivo: What Cell Culture Models Tell Us about Infectious Proteins.International journal of cell biology · 2013Review
- Prion disease and the innate immune system.Viruses · 2012Review
- N-glycans and glycosylphosphatidylinositol-anchor act on polarized sorting of mouse PrP(C) in Madin-Darby canine kidney cells.PloS one · 2011Article
- Glycosaminoglycan sulphation affects the seeded misfolding of a mutant prion protein.PloS one · 2010Article
- The physical relationship between infectivity and prion protein aggregates is strain-dependent.PLoS pathogens · 2010Article
- Infection of cell lines with experimental and natural ovine scrapie agents.Journal of virology · 2010Article
- Neuronal low-density lipoprotein receptor-related protein 1 binds and endocytoses prion fibrils via receptor cluster 4.Journal of cell science · 2010Article
- Getting a grip on prions: oligomers, amyloids, and pathological membrane interactions.Annual review of biochemistry · 2009Review
- Acute cellular uptake of abnormal prion protein is cell type and scrapie-strain independent.Virology · 2008Article
- Prion strain discrimination in cell culture: the cell panel assay.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the interactions of exogenous prions with an epithelial cell line inducibly expressing PrPc protein and permissive to infection by a sheep scrapie agent. We demonstrate that abnormal PrP (PrPSc) and prion infectivity are efficiently internalized in Rov cells, whether or not PrPc is expressed. At odds with earlier studies implicating cellular heparan sulfates in PrPSc internalization, we failed to find any involvement of such molecules in Rov cells, indicating that prions can enter target cells by several routes. We further show that PrPSc taken up in the absence of PrPc was unable to promote efficient prion multiplication once PrPc expression was restored in the cells. This observation argues that interaction of PrPSc with PrPc has to occur early, in a specific subcellular compartment(s), and is consistent with the view that the first prion multiplication events may occur at the cell surface.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.