ArticleNucleic acids research2007
Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Domain Mobility in the ORF2p Complex Revealed by Molecular Dynamics Simulations and Big Data Analysis.International journal of molecular sciences · 2024Article
- Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease.Nature communications · 2024Article
- Article
- Structural dissection of sequence recognition and catalytic mechanism of human LINE-1 endonuclease.Nucleic acids research · 2021Article
- Local features determine Ty3 targeting frequency at RNA polymerase III transcription start sites.Genome research · 2019Article
- Genome-wide de novo L1 Retrotransposition Connects Endonuclease Activity with Replication.Cell · 2019Article
- Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone.Malaria journal · 2018Article
- Cross-Kingdom Commonality of a Novel Insertion Signature of RTE-Related Short Retroposons.Genome biology and evolution · 2018Article
- Article
- Intact piRNA pathway prevents L1 mobilization in male meiosis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Insertion and deletion polymorphisms of the ancientMobile DNA · 2017Article
- Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8.Mobile DNA · 2017Article
- euL1db: the European database of L1HS retrotransposon insertions in humans.Nucleic acids research · 2015Article
- Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein.Mobile DNA · 2014Article
- L1 retrotransposition: The snap-velcro model and its consequences.Mobile genetic elements · 2014Article
- Article
- The specificity and flexibility of l1 reverse transcription priming at imperfect T-tracts.PLoS genetics · 2013Article
- Mammalian NUMT insertion is non-random.Nucleic acids research · 2012Article
- LINEs, SINEs and other retroelements: do birds of a feather flock together?Frontiers in bioscience (Landmark edition) · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of new L1 and Alu elements that presently account for approximately 28% of the human genome. L1-EN bears considerable technological interest, because its target selectivity may ultimately be engineered to allow the site-specific integration of DNA into defined genomic locations. Based on the crystal structure, we generated L1-EN mutants to analyze and manipulate DNA target site recognition. Crystal structures and their dynamic and functional analysis show entire loop grafts to be feasible, resulting in altered specificity, while individual point mutations do not change the nicking pattern of L1-EN. Structural parameters of the DNA target seem more important for recognition than the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro are less well defined than the respective integration site consensus in vivo. This suggests that additional factors other than the DNA nicking specificity of L1-EN contribute to the targeted integration of non-LTR retrotransposons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.