ArticleBiochemical pharmacology2007
Functional consequences of methionine oxidation of hERG potassium channels.
Article in Biochemical pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence.Scientific reports · 2023Article
- Methionine Sulfoxide Reductase B Regulates the Activity of Ascorbate Peroxidase of Banana Fruit.Antioxidants (Basel, Switzerland) · 2021Article
- Redox Regulation of the NOR Transcription Factor Is Involved in the Regulation of Fruit Ripening in Tomato.Plant physiology · 2020Article
- Defective protein repair under methionine sulfoxide A deletion drives autophagy and ARE-dependent gene transcription.Redox biology · 2018Article
- Preclinical study of a Kv11.1 potassium channel activator as antineoplastic approach for breast cancer.Oncotarget · 2018Article
- Genome-Wide Analysis of Genes Encoding Methionine-Rich Proteins in Arabidopsis and Soybean Suggesting Their Roles in the Adaptation of Plants to Abiotic Stress.International journal of genomics · 2016Article
- Regulation of protein function by reversible methionine oxidation and the role of selenoprotein MsrB1.Antioxidants & redox signaling · 2015Review
- PyTMs: a useful PyMOL plugin for modeling common post-translational modifications.BMC bioinformatics · 2014Article
- Oxidative modulation of voltage-gated potassium channels.Antioxidants & redox signaling · 2014Review
- Regulation of Kv4.2 A-Type Potassium Channels in HEK-293 Cells by Hypoxia.Frontiers in cellular neuroscience · 2014Article
- A Memory Molecule, Ca(2+)/Calmodulin-Dependent Protein Kinase II and Redox Stress; Key Factors for Arrhythmias in a Diseased Heart.Korean circulation journal · 2013Article
- Unusual case of severe arrhythmia developed after acute intoxication with tosylchloramide.BMC pharmacology & toxicology · 2013Article
- Metabolic stress, reactive oxygen species, and arrhythmia.Journal of molecular and cellular cardiology · 2012Review
- CaMKII in the cardiovascular system: sensing redox states.Physiological reviews · 2011Review
- Synergistic roles of Helicobacter pylori methionine sulfoxide reductase and GroEL in repairing oxidant-damaged catalase.The Journal of biological chemistry · 2011Article
- The biological significance of methionine sulfoxide stereochemistry.Free radical biology & medicine · 2011Review
- Cysteine 723 in the C-linker segment confers oxidative inhibition of hERG1 potassium channels.The Journal of physiology · 2010Article
- Functions and evolution of selenoprotein methionine sulfoxide reductases.Biochimica et biophysica acta · 2009Review
- Repolarization abnormalities and afterdepolarizations in a canine model of sudden cardiac death.American journal of physiology. Regulatory, integrative and comparative physiology · 2008Article
- Oxidation of multiple methionine residues impairs rapid sodium channel inactivation.Pflugers Archiv : European journal of physiology · 2008Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Reactive species oxidatively modify numerous proteins including ion channels. Oxidative sensitivity of ion channels is often conferred by amino acids containing sulfur atoms, such as cysteine and methionine. Functional consequences of oxidative modification of methionine in human ether à go-go related gene 1 (hERG1), which encodes cardiac I(Kr) channels, are unknown. Here we used chloramine-T (ChT), which preferentially oxidizes methionine, to examine the functional consequences of methionine oxidation of hERG channels stably expressed in a human embryonic kidney cell line (HEK 293) and native hERG channels in a human neuroblastoma cell line (SH-SY5Y). ChT (300 microM) significantly decreased whole-cell hERG current in both HEK 293 and SH-SY5Y cells. In HEK 293 cells, the effects of ChT on hERG current were time- and concentration-dependent, and were markedly attenuated in the presence of enzyme methionine sulfoxide reductase A that specifically repairs oxidized methionine. After treatment with ChT, the channel deactivation upon repolarization to -60 or -100 mV was significantly accelerated. The effect of ChT on channel activation kinetics was voltage-dependent; activation slowed during depolarization to +30 mV but accelerated during depolarization to 0 or -10mV. In contrast, the reversal potential, inactivation kinetics, and voltage-dependence of steady-state inactivation remained unaltered. Our results demonstrate that the redox status of methionine is an important modulator of hERG channel.
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