ArticleOncogene2008
A high-throughput screening for mammalian cell death effectors identifies the mitochondrial phosphate carrier as a regulator of cytochrome c release.
Article in Oncogene, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 105 citations in OpenAlex.
- Organic cation transporter 3 on neuronal mitochondria mediates MPPBMC biology · 2025Article
- MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity.Cell death & disease · 2025Article
- Dysfunction of Drosophila mitochondrial carrier homolog (Mtch) alters apoptosis and disturbs development.FEBS open bio · 2024Article
- CDK5 promotes apoptosis and attenuates chemoresistance in gastric cancer via E2F1 signaling.Cancer cell international · 2023Article
- Mitochondrial Carriers and Substrates Transport Network: A Lesson fromInternational journal of molecular sciences · 2021Review
- Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids.Nature communications · 2021Article
- Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma.Molecular medicine (Cambridge, Mass.) · 2021Article
- Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model.Scientific reports · 2021Article
- Polychemotherapy with Curcumin and Doxorubicin via Biological Nanoplatforms: Enhancing Antitumor Activity.Pharmaceutics · 2020Review
- The still uncertain identity of the channel-forming unit(s) of the mitochondrial permeability transition pore.Cell calcium · 2018Review
- Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis.BioMed research international · 2016Article
- CHZ868, a Type II JAK2 Inhibitor, Reverses Type I JAK Inhibitor Persistence and Demonstrates Efficacy in Myeloproliferative Neoplasms.Cancer cell · 2015Article
- Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition.Oncogene · 2015Review
- Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death.BMC cell biology · 2015Article
- Physiological and pathological roles of the mitochondrial permeability transition pore in the heart.Cell metabolism · 2015Review
- Genetic deletion of the mitochondrial phosphate carrier desensitizes the mitochondrial permeability transition pore and causes cardiomyopathy.Cell death and differentiation · 2014Article
- Systems biology of cisplatin resistance: past, present and future.Cell death & disease · 2014Review
- Novel insights into the mitochondrial permeability transition.Cell cycle (Georgetown, Tex.) · 2014Article
- The mitochondrial permeability transition pore and cancer: molecular mechanisms involved in cell death.Frontiers in oncology · 2014Review
- Oxidative stress and microRNAs in vascular diseases.International journal of molecular sciences · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional annotation of complex genomes requires the development of novel experimental platforms with increased capacity. Here, we describe a high-throughput system designed to identify cDNAs whose overexpression induces morphologically distinct cell death modalities. The methodology incorporates two robotized steps, and relies on coexpression of library clones with GFP to reveal the morphological features presented by the dying cells. By using this system we screened 135 000 cDNA clones and obtained 90 independent molecules. Interestingly, three death categories were identified, namely; apoptotic, vacuolated and autophagic. Among the pro-apoptotic clones, we found four members of the mitochondrial carrier family: the phosphate and adenine nucleotide (type 3) transporters, and the mitochondrial carrier homologs (MTCHs) 1 and 2. Expression of these molecules induced cytochrome c release and caspase-9-dependent death. One of them, the phosphate carrier, was able to interact with members of the permeability transition pore complex ANT1 and VDAC1, and its binding to ANT1 was stabilized in the presence of apoptotic activators. Depletion of this carrier by siRNA delayed cytochrome c mobilization and apoptosis. These results attribute a previously undescribed apoptotic function to the phosphate carrier and, more generally, suggest that a common property of various mitochondrial transporters was exploited during evolution to regulate apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.