ArticleFEMS yeast research2007
Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae.
Article in FEMS yeast research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Unremodeled GPI-anchored proteins at the plasma membrane trigger aberrant endocytosis.Life science alliance · 2025Article
- GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast.G3 (Bethesda, Md.) · 2024Article
- GPI-anchored Gas1 protein regulates cytosolic proteostasis in yeast.bioRxiv : the preprint server for biology · 2023Article
- Article
- Discovering the Influence of Microorganisms on Wine Color.Frontiers in microbiology · 2021Review
- A Theoretical Framework for Evolutionary Cell Biology.Journal of molecular biology · 2020Review
- Integration of Biochemical, Biophysical and Transcriptomics Data for Investigating the Structural and Nanomechanical Properties of the Yeast Cell Wall.Frontiers in microbiology · 2017Article
- SWATH-MS Glycoproteomics Reveals Consequences of Defects in the Glycosylation Machinery.Molecular & cellular proteomics : MCP · 2016Article
- The N-Linked Outer Chain Mannans and the Dfg5p and Dcw1p Endo-α-1,6-Mannanases Are Needed for Incorporation of Candida albicans Glycoproteins into the Cell Wall.Eukaryotic cell · 2015Article
- Cell wall-related bionumbers and bioestimates of Saccharomyces cerevisiae and Candida albicans.Eukaryotic cell · 2014Review
- Screening for glycosylphosphatidylinositol-modified cell wall proteins in Pichia pastoris and their recombinant expression on the cell surface.Applied and environmental microbiology · 2013Article
- Review
- The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections.BMC genomics · 2011Article
- A screen for deficiencies in GPI-anchorage of wall glycoproteins in yeast.Yeast (Chichester, England) · 2010Article
- Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.Molecular biology of the cell · 2009Article
- C-terminal signals regulate targeting of glycosylphosphatidylinositol-anchored proteins to the cell wall or plasma membrane in Candida albicans.Eukaryotic cell · 2008Article
- The cell wall of the human pathogen Candida glabrata: differential incorporation of novel adhesin-like wall proteins.Eukaryotic cell · 2008Article
- Assembly of the yeast cell wall. Crh1p and Crh2p act as transglycosylases in vivo and in vitro.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cell wall of yeast consists of an internal skeletal layer and an external layer of glycoproteins covalently linked to the stress-bearing polysaccharides. The cell wall protein (CWP) population consists of over 20 different proteins, and may vary in composition. We present two complementary methods for quantifying CWPs, based on isobaric tagging and tandem MS: (1) absolute quantitation of individual CWPs, allowing estimation of surface densities; and (2) relative quantitation of CWPs, allowing monitoring of the dynamics of the CWP population. For absolute quantitation, we selected a representative group of five proteins (Cwp1p, Crh1p, Scw4p, Gas1p, and Ecm33p), which had 67 x 10(3), 44 x 10(3), 38 x 10(3), 11 x 10(3) and 6.5 x 10(3) of wall-bound copies per cell, respectively. As Cwp1p is predominantly incorporated in the birth scar, this corresponds to a protein density of c. 22 x 10(3) copies microm(-2). For relative quantitation, we compared wild-type cells to gas1Delta cells, in which the cell wall integrity pathway is constitutively activated. The levels of Crh1p, Crh2p, Ecm33p, Gas5p, Pst1p and Pir3p increased about three- to fivefold, whereas the level of Scw4p was significantly decreased. We propose that our methods are widely applicable to other fungi.
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