ArticleJournal of bacteriology2007
G-protein control of the ribosome-associated stress response protein SpoT.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 83 citations in OpenAlex.
- Novel rRNA transcriptional activity of NhaR revealed by its growth recovery for theFrontiers in molecular biosciences · 2023Article
- Cellular perception of growth rate and the mechanistic origin of bacterial growth law.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Regulation ofFrontiers in microbiology · 2021Article
- SpoT Induces IntracellularmBio · 2020Article
- Activation of RelA by pppGpp as the basis for its differential toxicity over ppGpp inJournal of biosciences · 2020Article
- The Ribosome as a Switchboard for Bacterial Stress Response.Frontiers in microbiology · 2020Review
- Studies on the Regulation of (p)ppGpp Metabolism and Its Perturbation Through the Over-Expression of Nudix Hydrolases inFrontiers in microbiology · 2020Article
- The C-Terminal RRM/ACT Domain Is Crucial for Fine-Tuning the Activation of 'Long' RelA-SpoT Homolog Enzymes by Ribosomal Complexes.Frontiers in microbiology · 2020Article
- Make and break the alarmone: regulation of (p)ppGpp synthetase/hydrolase enzymes in bacteria.FEMS microbiology reviews · 2019Review
- The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.Nucleic acids research · 2018Article
- Escherichia coli translation strategies differ across carbon, nitrogen and phosphorus limitation conditions.Nature microbiology · 2018Article
- Guanosine tetra- and pentaphosphate increase antibiotic tolerance by reducing reactive oxygen species production inThe Journal of biological chemistry · 2018Article
- Monitoring of nutrient limitation in growing E. coli: a mathematical model of a ppGpp-based biosensor.BMC systems biology · 2017Article
- The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli.Biomolecules · 2017Article
- Structural and biochemical analysis ofThe Journal of biological chemistry · 2017Article
- The extended loops of ribosomal proteins uL4 and uL22 of Escherichia coli contribute to ribosome assembly and protein translation.Nucleic acids research · 2016Article
- The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target.BMC microbiology · 2015Article
- Many means to a common end: the intricacies of (p)ppGpp metabolism and its control of bacterial homeostasis.Journal of bacteriology · 2015Review
- The bacterial translation stress response.FEMS microbiology reviews · 2014Review
- Nitrogen stress response and stringent response are coupled in Escherichia coli.Nature communications · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial response to stress is controlled by two proteins, RelA and SpoT. RelA generates the alarmone (p)ppGpp under amino acid starvation, whereas SpoT is responsible for (p)ppGpp hydrolysis and for synthesis of (p)ppGpp under a variety of cellular stress conditions. It is widely accepted that RelA is associated with translating ribosomes. The cellular location of SpoT, however, has been controversial. SpoT physically interacts with the ribosome-associated GTPase CgtA, and we show here that, under an optimized salt condition, SpoT is also associated with a pre-50S particle. Analysis of spoT and cgtA mutants and strains overexpressing CgtA suggests that the ribosome associations of SpoT and CgtA are mutually independent. The steady-state level of (p)ppGpp is increased in a cgtA mutant, but the accumulation of (p)ppGpp during amino acid starvation is not affected, providing strong evidence that CgtA regulates the (p)ppGpp level during exponential growth but not during the stringent response. We show that CgtA is not associated with pre-50S particles during amino acid starvation, indicating that under these conditions in which (p)ppGpp accumulates, CgtA is not bound either to the pre-50S particle or to SpoT. We propose that, in addition to its role as a 50S assembly factor, CgtA promotes SpoT (p)ppGpp degradation activity on the ribosome and that the loss of CgtA from the ribosome is necessary for maximal (p)ppGpp accumulation under stress conditions. Intriguingly, we found that in the absence of spoT and relA, cgtA is still an essential gene in Escherichia coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.