ArticleJournal of bacteriology2007
Identification of Aeromonas veronii genes required for colonization of the medicinal leech, Hirudo verbana.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- How It All Begins: Bacterial Factors Mediating the Colonization of Invertebrate Hosts by Beneficial Symbionts.Microbiology and molecular biology reviews : MMBR · 2022Review
- Isolation of Skin Leukocytes Uncovers Phagocyte Inflammatory Responses During Induction and Resolution of Cutaneous Inflammation in Fish.Frontiers in immunology · 2021Article
- Closed Genome Sequence of Aeromonas veronii Strain Hm21, an Isolate from the Medicinal Leech Hirudo verbana.Microbiology resource announcements · 2020Article
- AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech.Journal of innate immunity · 2019Review
- Article
- Draft Genome Sequence of Aeromonas lusitana sp. nov. Strain DSM 24905Genome announcements · 2018Article
- Draft Genome Sequence of Aeromonas cavernicola sp. nov. DSM 24474Genome announcements · 2018Article
- Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.Science immunology · 2016Article
- Medicinal leech therapy and Aeromonas spp. infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- A Tale of Transmission: Aeromonas veronii Activity within Leech-Exuded Mucus.Applied and environmental microbiology · 2016Article
- Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential.Frontiers in microbiology · 2016Review
- Article
- High-throughput, signature-tagged mutagenic approach to identify novel virulence factors of Yersinia pestis CO92 in a mouse model of infection.Infection and immunity · 2015Article
- Identification of iron and heme utilization genes in Aeromonas and their role in the colonization of the leech digestive tract.Frontiers in microbiology · 2015Article
- Preventing infective complications following leech therapy: elimination of symbiotic Aeromonas spp. from the intestine of Hirudo verbana using antibiotic feeding.Surgical infections · 2014Article
- Draft Genome Sequence of Aeromonas veronii Hm21, a Symbiotic Isolate from the Medicinal Leech Digestive Tract.Genome announcements · 2013Article
- Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis.Microbiology (Reading, England) · 2013Article
- From in vitro to in vivo Models of Bacterial Biofilm-Related Infections.Pathogens (Basel, Switzerland) · 2013Review
- Knowing your friends: invertebrate innate immunity fosters beneficial bacterial symbioses.Nature reviews. Microbiology · 2012Review
- From environment to man: genome evolution and adaptation of human opportunistic bacterial pathogens.Genes · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most digestive tracts contain a complex consortium of beneficial microorganisms, making it challenging to tease apart the molecular interactions between symbiont and host. The digestive tract of Hirudo verbana, the medicinal leech, is an ideal model system because it harbors a simple microbial community in the crop, comprising the genetically amenable Aeromonas veronii and a Rikenella-like bacterium. Signature-tagged mutagenesis (STM) was used to identify genes required for digestive tract colonization. Of 3,850 transposon (Tn) mutants screened, 46 were identified as colonization mutants. Previously we determined that the complement system of the ingested blood remained active inside the crop and prevented serum-sensitive mutants from colonizing. The identification of 26 serum-sensitive mutants indicated a successful screen. The remaining 20 serum-resistant mutants are described in this study and revealed new insights into symbiont-host interactions. An in vivo competition assay compared the colonization levels of the mutants to that of a wild-type competitor. Attenuated colonization mutants were grouped into five classes: surface modification, regulatory, nutritional, host interaction, and unknown function. One STM mutant, JG736, with a Tn insertion in lpp, encoding Braun's lipoprotein, was characterized in detail. This mutant had a >25,000-fold colonization defect relative to colonization by the wild-type strain at 72 h and, in vitro, an increased sensitivity to sodium dodecyl sulfate, suggesting the presence of an additional antimicrobial property in the crop. The classes of genes identified in this study are consistent with findings from previous STM studies involving pathogenic bacteria, suggesting parallel molecular requirements for beneficial and pathogenic host colonization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.