ArticleAnnals of botany2007
Highly efficient virus-induced gene silencing (VIGS) in California poppy (Eschscholzia californica): an evaluation of VIGS as a strategy to obtain functional data from non-model plants.
Article in Annals of botany, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 138 citations in OpenAlex.
- Gene and genome duplications have contrasting impacts on biosynthetic and flower developmental pathways in California poppy.The Plant cell · 2026Article
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- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- A cornucopia of diversity-Ranunculales as a model lineage.Journal of experimental botany · 2024Review
- Applications of Virus-Induced Gene Silencing in Cotton.Plants (Basel, Switzerland) · 2024Review
- California poppy (Frontiers in plant science · 2023Review
- Tissue-Specific RNA-Seq Analysis and Identification of Receptor-Like Proteins Related to Plant Growth inPlants (Basel, Switzerland) · 2021Article
- Virus-induced gene silencing as a tool for functional studies inApplications in plant sciences · 2021Article
- The Role of INAPERTURATE POLLEN1 as a Pollen Aperture Factor Is Conserved in the Basal EudicotFrontiers in plant science · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- Independent evolution of rosmarinic acid biosynthesis in two sister families under the Lamiids clade of flowering plants.The Journal of biological chemistry · 2019Article
- Article
- Article
- Insights into carotenoid accumulation using VIGS to block different steps of carotenoid biosynthesis in petals of California poppy.Plant cell reports · 2018Article
- Herbal genomics as tools for dissecting new metabolic pathways of unexplored medicinal plants and drug discovery.Biochimie open · 2018Review
- Tobacco rattle virus-inducedPeerJ · 2018Article
- Development of basic technologies for improvement of breeding and cultivation of Japanese gentian.Breeding science · 2018Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsEschscholzia californica (California poppy) is an emerging model plant for 'evo-devo' studies from the basal eudicot clade of Papaveraceae. California poppy has a relatively small genome, a short life cycle and, most importantly, it is amenable for transformation. However, since this transformation protocol is time consuming, virus-induced gene silencing (VIGS) was evaluated as a fast method to obtain functional data for California poppy genes.
methodsCommercially available California poppy plants were infiltrated with Agrobacterium tumefaciens carrying the tobacco rattle virus plasmids pTRV1 and pTRV2. pTRV2 contained part of the eschscholzia Phytoene Desaturase (EcPDS) gene whose loss of function results in photobleaching of the green parts of the plant and in a lack of floral coloration. The degree and duration of these symptoms was evaluated for vegetative rosettes and plants in flower. KEY
resultsIt is shown that VIGS is able to effectively down-regulate the EcPDS gene in eschscholzia. Various degrees of silencing were observed starting <2 weeks after infiltration with Agrobacterium tumefaciens in 92 % of the plants. Tissue with silencing symptoms also showed complete or strong reduction of EcPDS transcripts. Strong silencing resulted in almost completely white petals, fruits, shoots and leaves. Plants with a strong degree of silencing will eventually die off; however, others are able to produce EcPDS gene product even after a strong initial silencing and will recover. Silencing was found to be not always systemic, but was often restricted to certain organs or parts of organs.
conclusionsVIGS is an effective, fast and transient method to down-regulate gene expression in eschscholzia. It serves well to detect prominent phenotypes which may become obvious even if some target gene transcript remains in the plant tissue. However, subtle phenotypes will be more difficult to detect, as extremely strong silencing effects occur in <10 % of all flowers from infected plants.
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