ArticleCurrent eye research2007
Primary culture of porcine nonpigmented ciliary epithelium.
Article in Current eye research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Mechanical Stretch Activates TRPV4 and Hemichannel Responses in the Nonpigmented Ciliary Epithelium.International journal of molecular sciences · 2023Article
- The vital role for nitric oxide in intraocular pressure homeostasis.Progress in retinal and eye research · 2021Review
- A Theoretical Approach for the Electrochemical Characterization of Ciliary Epithelium.Life (Basel, Switzerland) · 2020Article
- Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium.Journal of cellular physiology · 2017Article
- Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632.Molecular vision · 2015Article
- Nitric oxide regulation of Na, K-ATPase activity in ocular ciliary epithelium involves Src family kinase.Journal of cellular physiology · 2014Article
- Nonpigmented ciliary epithelial cells respond to acetazolamide by a soluble adenylyl cyclase mechanism.Investigative ophthalmology & visual science · 2014Article
- Connexins form functional hemichannels in porcine ciliary epithelium.Experimental eye research · 2014Article
- DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism.American journal of physiology. Cell physiology · 2013Article
- Responses of sodium-hydrogen exchange to nitric oxide in porcine cultured nonpigmented ciliary epithelium.Investigative ophthalmology & visual science · 2009Article
- Localization of multidrug resistance-associated protein 2 in the nonpigmented ciliary epithelium of the eye.The Journal of pharmacology and experimental therapeutics · 2009Article
- Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.Investigative ophthalmology & visual science · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposePrimary culture of nonpigmented ciliary epithelium (NPE) has proved difficult in the past. Here we report development of a method of growing and maintaining primary cultures of NPE from porcine eye. Studies were conducted to confirm that the cultured NPE expressed proteins characteristic of native NPE.
methodsIntact rings of NPE were isolated from adult pig eyes. A mixture of hyaluronidase and collagenase was used to detach the cells from the basement membrane and vitreous. Dispersed cells were seeded at high density and grown in DMEM with 20% fetal bovine serum under 5% CO2 and 95% air. Protein expression was examined by immunohistochemistry and immunoblot analysis.
resultsNPE cells were grown in primary culture and maintained up to 10th passage. Analysis of the ciliary body showed three Na, K-ATPase isoforms (alpha 1, alpha 2, alpha 3) and three nitric oxide synthase isoforms (eNOS, nNOS, iNOS) enriched in the NPE layer but weaker or absent in the PE layer. Each of these proteins as well as the tight junction-specific protein ZO-1 was detected in the cultured NPE.
conclusionsWe developed a simple and reliable way to isolate, culture, and maintain NPE cells from porcine eyes. Success of the method hinged on our ability to isolate pure NPE in large number, detach the cells from the vitreous, and seed the cells at high density. The cultured cells express several proteins that are characteristic of native NPE. NPE cells cultured in this way may prove to be valuable for the study of ciliary body function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.