ArticleBMC genomics2007
Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- The Role of Biofilm-Derived Compounds in Microbial and Protozoan Interactions.Microorganisms · 2025Review
- Pathogenicity and virulence ofVirulence · 2023Review
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Attenuation of In Vitro and In Vivo Virulence Is Associated with Repression of Gene Expression of AIG1 Gene inPathogens (Basel, Switzerland) · 2023Article
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
- Advances inFrontiers in microbiology · 2019Review
- Comparative effects of histone deacetylases inhibitors and resveratrol on Trypanosoma cruzi replication, differentiation, infectivity and gene expression.International journal for parasitology. Drugs and drug resistance · 2017Article
- Epigenome mapping highlights chromatin-mediated gene regulation in the protozoan parasite Trichomonas vaginalis.Scientific reports · 2017Article
- Technical advances in trigger-induced RNA interference gene silencing in the parasite Entamoeba histolytica.International journal for parasitology · 2016Article
- Epigenetic origin of adaptive phenotypic variants in the human blood fluke Schistosoma mansoni.Epigenetics & chromatin · 2016Article
- A genomewide overexpression screen identifies genes involved in the phosphatidylinositol 3-kinase pathway in the human protozoan parasite Entamoeba histolytica.Eukaryotic cell · 2014Article
- Robust gene silencing mediated by antisense small RNAs in the pathogenic protist Entamoeba histolytica.Nucleic acids research · 2013Article
- Exposure to histone deacetylase inhibitors during Pavlovian conditioning enhances subsequent cue-induced reinstatement of operant behavior.Behavioural pharmacology · 2013Article
- Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes.BMC genomics · 2013Article
- A detoxifying oxygen reductase in the anaerobic protozoan Entamoeba histolytica.Eukaryotic cell · 2012Article
- A genome-wide over-expression screen identifies genes involved in phagocytosis in the human protozoan parasite, Entamoeba histolytica.PloS one · 2012Article
- A developmentally regulated Myb domain protein regulates expression of a subset of stage-specific genes in Entamoeba histolytica.Cellular microbiology · 2009Article
- Development of the Gateway system for cloning and expressing genes in Entamoeba histolytica.Parasitology international · 2009Article
- Using differential gene expression to study Entamoeba histolytica pathogenesis.Trends in parasitology · 2009Review
- Recent insights into Entamoeba development: identification of transcriptional networks associated with stage conversion.International journal for parasitology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundHistone modification regulates chromatin structure and influences gene expression associated with diverse biological functions including cellular differentiation, cancer, maintenance of genome architecture, and pathogen virulence. In Entamoeba, a deep-branching eukaryote, short chain fatty acids (SCFA) affect histone acetylation and parasite development. Additionally, a number of active histone modifying enzymes have been identified in the parasite genome. However, the overall extent of gene regulation tied to histone acetylation is not known.
resultsIn order to identify the genome-wide effects of histone acetylation in regulating E. histolytica gene expression, we used whole-genome expression profiling of parasites treated with SCFA and Trichostatin A (TSA). Despite significant changes in histone acetylation patterns, exposure of parasites to SCFA resulted in minimal transcriptional changes (11 out of 9,435 genes transcriptionally regulated). In contrast, exposure to TSA, a more specific inhibitor of histone deacetylases, significantly affected transcription of 163 genes (122 genes upregulated and 41 genes downregulated). Genes modulated by TSA were not regulated by treatment with 5-Azacytidine, an inhibitor of DNA-methyltransferase, indicating that in E. histolytica the crosstalk between DNA methylation and histone modification is not substantial. However, the set of genes regulated by TSA overlapped substantially with genes regulated during parasite development: 73/122 genes upregulated by TSA exposure were upregulated in E. histolytica cysts (p-value = 6 x 10(-53)) and 15/41 genes downregulated by TSA exposure were downregulated in E. histolytica cysts (p-value = 3 x 10(-7)).
conclusionThis work represents the first genome-wide analysis of histone acetylation and its effects on gene expression in E. histolytica. The data indicate that SCFAs, despite their ability to influence histone acetylation, have minimal effects on gene transcription in cultured parasites. In contrast, the effect of TSA on E. histolytica gene expression is more substantial and includes genes involved in the encystation pathway. These observations will allow further dissection of the effects of histone acetylation and the genetic pathways regulating stage conversion in this pathogenic parasite.
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