ArticleMolecular and biochemical parasitology2007
Characterization of the Trypanosoma brucei cap hypermethylase Tgs1.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Loss of the RNA trimethylguanosine cap is compatible with nuclear accumulation of spliceosomal snRNAs but not pre-mRNA splicing or snRNA processing during animal development.PLoS genetics · 2020Article
- Review
- Article
- Differential Subcellular Localization of Leishmania Alba-Domain Proteins throughout the Parasite Development.PloS one · 2015Article
- Multiplicity of 5' cap structures present on short RNAs.PloS one · 2014Article
- RNA methyltransferases involved in 5' cap biosynthesis.RNA biology · 2014Review
- Evolutionary conservation and diversification of the translation initiation apparatus in trypanosomatids.Comparative and functional genomics · 2012Article
- Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7.Molecular and biochemical parasitology · 2011Article
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- 5' and 3' end modifications of spliceosomal RNAs in Plasmodium falciparum.Molecular biology reports · 2010Article
- Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1.Nucleic acids research · 2009Article
- Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex.Molecular and cellular biology · 2009Article
- The divergent eukaryote Trichomonas vaginalis has an m7G cap methyltransferase capable of a single N2 methylation.Nucleic acids research · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many U-snRNAs contain a hypermodified 2,2,7-trimethylguanosine (TMG) cap structure, which is formed by post-transcriptional methylation of an m(7)G cap. At present, little is known about the maturation of U-snRNAs in trypanosomes. The current evidence is consistent with the primary transcript containing an m(7)G moiety, but it is not clear whether the conversion of m(7)G to TMG takes place in the cytoplasm or in the nucleus. To address this issue, we characterized the Trypanosoma brucei homologue of the trimethylguanosine synthase (TbTgs1), a 28kDa protein, which is mainly composed of the conserved catalytic domain and lacks a large N-terminal domain present in higher eukaryotes. A GFP fusion with TbTgs1 revealed that this protein localizes throughout the nucleoplasm, as well as in one or two dots outside the nucleolus and RNAi-mediated downregulation of TbTgs1 suggests that this protein is responsible for hypermethylation of the m(7)G cap of both snRNAs and snoRNAs.
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