ArticleProceedings of the National Academy of Sciences of the United States of America2007
Exploring lignification in conifers by silencing hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase in Pinus radiata.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 159 citations in OpenAlex.
- Completing the acylation landscape in plants: GDSL enzymes join the BAHD and SCPL acyltransferase families.Journal of experimental botany · 2026Review
- Genome-wide identification of HCT gene family in sugarcane (Saccharum spp. hybrid) and characterization of putative cis-elements in gene regulation.BMC genomics · 2026Article
- Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Plants (Basel, Switzerland) · 2024Article
- Exogenous methyl jasmonate and cytokinin antagonistically regulate lignin biosynthesis by mediating CsHCT expression in Camellia sinensis.Protoplasma · 2023Article
- Activity of Cytosolic Ascorbate Peroxidase (APX) fromInternational journal of molecular sciences · 2023Article
- Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Proteomic and metabolic disturbances in lignin-modified Brachypodium distachyon.The Plant cell · 2022Article
- Expression of a Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferase 4 Gene fromInternational journal of molecular sciences · 2022Article
- Identification and characterization of a set of monocot BAHD monolignol transferases.Plant physiology · 2022Article
- REPRISAL: mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis.Plant physiology · 2022Article
- Comparative analysis of differential gene expression indicates divergence in ontogenetic strategies of leaves in two conifer genera.Ecology and evolution · 2022Article
- Transcriptome and metabolome analyses provide insights into the relevance of pericarp thickness variations inFrontiers in plant science · 2022Article
- Transcriptome and metabolome analyses reveal phenotype formation differences between russet and non-russet apples.Frontiers in plant science · 2022Article
- Relationship between the Phenylpropanoid Pathway and Dwarfism ofInternational journal of molecular sciences · 2021Article
- Review
- Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes.The Plant cell · 2021Article
- Localised laccase activity modulates distribution of lignin polymers in gymnosperm compression wood.The New phytologist · 2021Article
- High-Throughput Sequencing Reveals the Regulatory Networks of Transcriptome and Small RNAs During the Defense AgainstFrontiers in plant science · 2021Article
- Chemical and Structural Responses to DownregulatedFrontiers in plant science · 2021Article
- Phylogenetic Occurrence of the Phenylpropanoid Pathway and Lignin Biosynthesis in Plants.Frontiers in plant science · 2021Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enzyme hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase (HCT) is involved in the production of methoxylated monolignols that are precursors to guaiacyl and syringyl lignin in angiosperm species. We identified and cloned a putative HCT gene from Pinus radiata, a coniferous gymnosperm that does not produce syringyl lignin. This gene was up-regulated during tracheary element (TE) formation in P. radiata cell cultures and showed 72.6% identity to the amino acid sequence of the Nicotiana tabacum HCT isolated earlier. RNAi-mediated silencing of the putative HCT gene had a strong impact on lignin content, monolignol composition, and interunit linkage distribution. AcBr assays revealed an up to 42% reduction in lignin content in TEs. Pyrolysis-GC/MS, thioacidolysis, and NMR detected substantial changes in lignin composition. Most notable was the rise of p-hydroxyphenyl units released by thioacidolysis, which increased from trace amounts in WT controls to up to 31% in transgenics. Two-dimensional 13C-1H correlative NMR confirmed the increase in p-hydroxyphenyl units in the transgenics and revealed structural differences, including an increase in resinols, a reduction in dibenzodioxocins, and the presence of glycerol end groups. The observed modifications in silenced transgenics validate the targeted gene as being associated with lignin biosynthesis in P. radiata and thus likely to encode HCT. This enzyme therefore represents the metabolic entry point leading to the biosynthesis of methoxylated phenylpropanoids in angiosperm species and coniferous gymnosperms such as P. radiata.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.