ArticleProceedings of the National Academy of Sciences of the United States of America2007
Extracellular structure of polysialic acid explored by on cell solution NMR.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity.FEBS letters · 2025Article
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
- Synthetic Approaches to Break the Chemical Shift Degeneracy of Glycans.Chembiochem : a European journal of chemical biology · 2022Article
- Rational identification and characterisation of peptide ligands for targeting polysialic acid.Scientific reports · 2020Article
- The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding.Methods in enzymology · 2019Article
- Predicting the Structures of Glycans, Glycoproteins, and Their Complexes.Chemical reviews · 2018Review
- The β-reducing end in α(2-8)-polysialic acid constitutes a unique structural motif.Glycobiology · 2017Article
- Insights from NMR Spectroscopy into the Conformational Properties of Man-9 and Its Recognition by Two HIV Binding Proteins.Chembiochem : a European journal of chemical biology · 2017Article
- Bexsero® chronicle.Pathogens and global health · 2014Review
- Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration.Physiological reviews · 2014Review
- Membrane potential-dependent binding of polysialic acid to lipid monolayers and bilayers.Cellular & molecular biology letters · 2013Article
- Sialic acids siglec interaction: a unique strategy to circumvent innate immune response by pathogens.The Indian journal of medical research · 2013Review
- Sialyldisaccharide conformations: a molecular dynamics perspective.Journal of computer-aided molecular design · 2012Article
- NMR detection and characterization of sialylated glycoproteins and cell surface polysaccharides.Journal of biomolecular NMR · 2011Article
- Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins.Journal of biomolecular NMR · 2010Review
- Early molecular-recognition events in the synthesis and export of group 2 capsular polysaccharides.Microbiology (Reading, England) · 2009Review
- The conformational properties of methyl alpha-(2,8)-di/trisialosides and their N-acyl analogues: implications for anti-Neisseria meningitidis B vaccine design.Biochemistry · 2008Article
- 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I.Journal of the American Chemical Society · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The capsular polysaccharide of the pathogens Neisseria meningitidis serogroup B and of Escherichia coli K1, alpha(2 --> 8) polysialic acid (PSA), is unusual, because when injected into adult humans, it generates little or no antibody. In contrast, people infected with these pathogens generate specific serum antibodies. A structural study on cells is used to address this anomaly by characterizing antigen structures in vivo. We introduce on cell multidimensional solution NMR spectroscopy for direct observation of PSA on E. coli bacteria. Using 13C,15N-labeled PSA, we applied a combination of heteronuclear NMR methods, such as heteronuclear single quantum coherence, HNCA, and HNCO, in vivo. Analysis reveals that free and cell-bound PSA are structurally similar, indicating that the poor immunogenicity of PSA is not due to major structural differences between cells and purified PSA. The 13C linewidths of PSA on cells are 2 to 3 times larger than the corresponding ones in free PSA. The possible implications of the differences between free and on cell PSA are discussed. In addition, we demonstrate the suitability of the method for in vivo kinetic studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.