ArticleMolecular microbiology2007
Neisseria gonorrhoeae O-linked pilin glycosylation: functional analyses define both the biosynthetic pathway and glycan structure.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 122 citations in OpenAlex.
- Saccharibacteria deploy two distinct type IV pili, driving episymbiosis, host competition, and twitching motility.The ISME journal · 2025Article
- Pilin antigenic variants impact gonococcal lifestyle and antibiotic tolerance by modulating interbacterial forces.PLoS biology · 2025Article
- Saccharibacteria deploy two distinct Type IV pili, driving episymbiosis, host competition, and twitching motility.bioRxiv : the preprint server for biology · 2024Article
- Sweet complexity:Frontiers in cellular and infection microbiology · 2024Review
- High-Frequency Changes in Pilin Glycosylation Patterns duringACS infectious diseases · 2023Article
- Genetic, Functional, and Immunogenic Analyses of theJournal of bacteriology · 2023Article
- Sculpting the BacterialmBio · 2022Article
- Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development.Environmental microbiology reports · 2022Article
- Diverse functions for acyltransferase-3 proteins in the modification of bacterial cell surfaces.Microbiology (Reading, England) · 2022Article
- Article
- Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system.PLoS genetics · 2019Article
- Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.Biophysical journal · 2019Article
- A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host.Nature communications · 2019Article
- The structure of PilA fromThe Journal of biological chemistry · 2019Article
- A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes inFrontiers in microbiology · 2019Article
- Disrupted Synthesis of a Di-Journal of bacteriology · 2019Article
- Genotypic and Phenotypic Characterization of theJournal of bacteriology · 2018Article
- How Sweet Are Our Gut Beneficial Bacteria? A Focus on Protein Glycosylation in Lactobacillus.International journal of molecular sciences · 2018Review
- Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the HalophileFrontiers in microbiology · 2018Article
- Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution.Structure (London, England : 1993) · 2017Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neisseria gonorrhoeae expresses an O-linked protein glycosylation pathway that targets PilE, the major pilin subunit protein of the Type IV pilus colonization factor. Efforts to define glycan structure and thus the functions of pilin glycosylation (Pgl) components at the molecular level have been hindered by the lack of sensitive methodologies. Here, we utilized a 'top-down' mass spectrometric approach to characterize glycan status using intact pilin protein from isogenic mutants. These structural data enabled us to directly infer the function of six components required for pilin glycosylation and to define the glycan repertoire of strain N400. Additionally, we found that the N. gonorrhoeae pilin glycan is O-acetylated, and identified an enzyme essential for this unique modification. We also identified the N. gonorrhoeae pilin oligosaccharyltransferase using bioinformatics and confirmed its role in pilin glycosylation by directed mutagenesis. Finally, we examined the effects of expressing the PglA glycosyltransferase from the Campylobacter jejuni N-linked glycosylation system that adds N-acetylgalactosamine onto undecaprenylpyrophosphate-linked bacillosamine. The results indicate that the C. jejuni and N. gonorrhoeae pathways can interact in the synthesis of O-linked di- and trisaccharides, and therefore provide the first experimental evidence that biosynthesis of the N. gonorrhoeae pilin glycan involves a lipid-linked oligosaccharide precursor. Together, these findings underpin more detailed studies of pilin glycosylation biology in both N. gonorrhoeae and N. meningitidis, and demonstrate how components of bacterial O- and N-linked pathways can be combined in novel glycoengineering strategies.
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