ArticlePLoS biology2007
Rb-mediated neuronal differentiation through cell-cycle-independent regulation of E2f3a.
Article in PLoS biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- Article
- Conditional Immortalization Using SV40 Large T Antigen and Its Effects on Induced Pluripotent Stem Cell Differentiation Toward Retinal Progenitor Cells.Stem cells and development · 2025Article
- The gut microbiota regulates diabetic retinopathy in adult rats.Frontiers in microbiology · 2025Article
- Defining morphologically and genetically distinct GABAergic/cholinergic amacrine cell subtypes in the vertebrate retina.PLoS biology · 2024Article
- Hyperglycemia-independent neonatal streptozotocin-induced retinopathy (NSIR) in rats.Frontiers in pharmacology · 2024Article
- Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons.Communications biology · 2022Article
- Long Non-coding RNA FEZF1-AS1 Promotes Growth and Reduces Apoptosis Through Regulation of miR-363-3p/PAX6 Axis in Retinoblastoma.Biochemical genetics · 2021Article
- Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma.JCI insight · 2019Article
- Rb is required for retinal angiogenesis and lamination.Cell death & disease · 2018Article
- miR-145 regulates the proliferation and apoptosis of Y79 human retinoblastoma cells by targeting IGF-1R.International journal of clinical and experimental pathology · 2018Article
- E2f1 mediates high glucose-induced neuronal death in cultured mouse retinal explants.Cell cycle (Georgetown, Tex.) · 2017Article
- Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells.International journal of molecular medicine · 2017Article
- Retinoblastoma cells activate the AKT pathway and are vulnerable to the PI3K/mTOR inhibitor NVP-BEZ235.Oncotarget · 2017Article
- Conservation and divergence of C-terminal domain structure in the retinoblastoma protein family.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Genome-wide analysis reveals NRP1 as a direct HIF1α-E2F7 target in the regulation of motorneuron guidance in vivo.Nucleic acids research · 2016Article
- Tissue-specific targeting of cell fate regulatory genes by E2f factors.Cell death and differentiation · 2016Article
- Coordinating progenitor cell cycle exit and differentiation in the developing vertebrate retina.Neurogenesis (Austin, Tex.) · 2016Article
- Control of the Cell Cycle in Adult Neurogenesis and its Relation with Physical Exercise.Brain plasticity (Amsterdam, Netherlands) · 2015Review
- Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas.Molecular cancer · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has long been known that loss of the retinoblastoma protein (Rb) perturbs neural differentiation, but the underlying mechanism has never been solved. Rb absence impairs cell cycle exit and triggers death of some neurons, so differentiation defects may well be indirect. Indeed, we show that abnormalities in both differentiation and light-evoked electrophysiological responses in Rb-deficient retinal cells are rescued when ectopic division and apoptosis are blocked specifically by deleting E2f transcription factor (E2f) 1. However, comprehensive cell-type analysis of the rescued double-null retina exposed cell-cycle-independent differentiation defects specifically in starburst amacrine cells (SACs), cholinergic interneurons critical in direction selectivity and developmentally important rhythmic bursts. Typically, Rb is thought to block division by repressing E2fs, but to promote differentiation by potentiating tissue-specific factors. Remarkably, however, Rb promotes SAC differentiation by inhibiting E2f3 activity. Two E2f3 isoforms exist, and we find both in the developing retina, although intriguingly they show distinct subcellular distribution. E2f3b is thought to mediate Rb function in quiescent cells. However, in what is to our knowledge the first work to dissect E2f isoform function in vivo we show that Rb promotes SAC differentiation through E2f3a. These data reveal a mechanism through which Rb regulates neural differentiation directly, and, unexpectedly, it involves inhibition of E2f3a, not potentiation of tissue-specific factors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.