ArticleMolecular and cellular biology2007
The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Trypanosome mRNA recapping is triggered by hypermethylation originating from cap 4.Nucleic acids research · 2024Article
- CMTr mediated 2'-RNA (New York, N.Y.) · 2022Article
- Enzymatic Assays to Explore Viral mRNA Capping Machinery.Chembiochem : a European journal of chemical biology · 2021Review
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- The Role of Cytoplasmic mRNA Cap-Binding Protein Complexes in Trypanosoma brucei and Other Trypanosomatids.Pathogens (Basel, Switzerland) · 2017Review
- The spliceosomal PRP19 complex of trypanosomes.Molecular microbiology · 2015Article
- RNA methyltransferases involved in 5' cap biosynthesis.RNA biology · 2014Review
- Structural analysis of human 2'-O-ribose methyltransferases involved in mRNA cap structure formation.Nature communications · 2014Article
- Trypanosome cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of RNA polymerase II subunit RPB1.Molecular and cellular biology · 2013Article
- 2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family.Nucleic acids research · 2011Article
- Characterization of hMTr1, a human Cap1 2'-O-ribose methyltransferase.The Journal of biological chemistry · 2010Article
- Analysis of spliceosomal proteins in Trypanosomatids reveals novel functions in mRNA processing.The Journal of biological chemistry · 2010Article
- Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins.RNA (New York, N.Y.) · 2009Article
- Hypermethylated cap 4 maximizes Trypanosoma brucei translation.Molecular microbiology · 2009Article
- Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex.Molecular and cellular biology · 2009Article
- Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.Eukaryotic cell · 2009Article
- Kinetoplastid genomics: the thin end of the wedge.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2008Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
mRNA cap 1 2'-O-ribose methylation is a widespread modification that is implicated in processing, trafficking, and translational control in eukaryotic systems. The eukaryotic enzyme has yet to be identified. In kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic precursors conveys a hypermethylated cap 4, including a cap 0 m7G and seven additional methylations on the first 4 nucleotides, to all nuclear mRNAs. We report the first eukaryotic cap 1 2'-O-ribose methyltransferase, TbMTr1, a member of a conserved family of viral and eukaryotic enzymes. Recombinant TbMTr1 methylates the ribose of the first nucleotide of an m7G-capped substrate. Knockdowns and null mutants of TbMTr1 in Trypanosoma brucei grow normally, with loss of 2'-O-ribose methylation at cap 1 on substrate SL RNA and U1 small nuclear RNA. TbMTr1-null cells have an accumulation of cap 0 substrate without further methylation, while spliced mRNA is modified efficiently at position 4 in the absence of 2'-O-ribose methylation at position 1; downstream cap 4 methylations are independent of cap 1. Based on TbMTr1-green fluorescent protein localization, 2'-O-ribose methylation at position 1 occurs in the nucleus. Accumulation of 3'-extended SL RNA substrate indicates a delay in processing and suggests a synergistic role for cap 1 in maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.