ArticleBMC molecular biology2007
Vertebrate DM domain proteins bind similar DNA sequences and can heterodimerize on DNA.
Article in BMC molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 97 citations in OpenAlex.
- Post-translational modification of proteins in the human testis development pathway.Human reproduction update · 2026Review
- A panoramic view of the expression and function of the Doublesex/DMRT gene family inScience advances · 2026Article
- Sex-Specific doublesex Regulation Targeting the Color-Patterning Gene h Underlies the Evolution of Wing Sexual Dimorphism in the Harlequin Ladybug Harmonia axyridis.Evolution & development · 2026Article
- Sex-specific functional evolution of Dmrt1 in African clawed frogs (Xenopus), and the importance of genetic tipping points in developmental biology.PLoS genetics · 2026Article
- The SMARCA5-DMRT1 Pioneer Complex Establishes Epigenetic Priming to Direct Male Germline Development.Research square · 2025Article
- Novel Insights into Emx2 and Dmrta2 Cooperation during Cortex Development and Evidence for Dmrta2 Function in the Choroid Plexus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex.Development (Cambridge, England) · 2025Article
- The Interaction of DMRTA2 with HSP90β Inhibits p53 Ubiquitination and Activates the p53 Pathway to Suppress the Malignant Progression of Non-Small-Cell Lung Cancer.Current issues in molecular biology · 2025Article
- Evidence That Dmrta2 Acts through Repression ofeNeuro · 2025Article
- Roles of a Y-Linked iDmrt1 Paralogue and Insulin-like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster,International journal of molecular sciences · 2025Article
- Diminished DNA binding affinity of DMRT1 caused by heterozygous DM domain mutations is a cause of male infertility.Human molecular genetics · 2025Article
- Analyses of theFrontiers in physiology · 2023Article
- Neofunctionalization of a Noncoding Portion of a DNA Transposon in the Coding Region of the Chimerical Sex-Determining Gene dm-W in Xenopus Frogs.Molecular biology and evolution · 2022Article
- DMRT Transcription Factors in the Control of Nervous System Sexual Differentiation.Frontiers in neuroanatomy · 2022Review
- Genetic Regulation of Avian Testis Development.Genes · 2021Review
- Identification of Sex-Related Genes from the Three-Spot Swimming CrabAnimals : an open access journal from MDPI · 2021Article
- The conserved sex regulator DMRT1 recruits SOX9 in sexual cell fate reprogramming.Nucleic acids research · 2021Article
- Cloning and characterization of rec8 gene in orange-spotted grouper (Epinephelus coioides) and Dmrt1 regulation of rec8 promoter activity.Fish physiology and biochemistry · 2021Article
- Dmrt2 promotes transition of endochondral bone formation by linking Sox9 and Runx2.Communications biology · 2021Article
- Multiple Functions of theFrontiers in neuroscience · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe DM domain is a zinc finger-like DNA binding motif first identified in the sexual regulatory proteins Doublesex (DSX) and MAB-3, and is widely conserved among metazoans. DM domain proteins regulate sexual differentiation in at least three phyla and also control other aspects of development, including vertebrate segmentation. Most DM domain proteins share little similarity outside the DM domain. DSX and MAB-3 bind partially overlapping DNA sequences, and DSX has been shown to interact with DNA via the minor groove without inducing DNA bending. DSX and MAB-3 exhibit unusually high DNA sequence specificity relative to other minor groove binding proteins. No detailed analysis of DNA binding by the seven vertebrate DM domain proteins, DMRT1-DMRT7 has been reported, and thus it is unknown whether they recognize similar or diverse DNA sequences.
resultsWe used a random oligonucleotide in vitro selection method to determine DNA binding sites for six of the seven proteins. These proteins selected sites resembling that of DSX despite differences in the sequence of the DM domain recognition helix, but they varied in binding efficiency and in preferences for particular nucleotides, and some behaved anomalously in gel mobility shift assays. DMRT1 protein from mouse testis extracts binds the sequence we determined, and the DMRT proteins can bind their in vitro-defined sites in transfected cells. We also find that some DMRT proteins can bind DNA as heterodimers.
conclusionOur results suggest that target gene specificity of the DMRT proteins does not derive exclusively from major differences in DNA binding specificity. Instead target specificity may come from more subtle differences in DNA binding preference between different homodimers, together with differences in binding specificity between homodimers versus heterodimers.
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