ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2007
Towards map-based cloning: fine mapping of a recessive genic male-sterile gene (BnMs2) in Brassica napus L. and syntenic region identification based on the Arabidopsis thaliana genome sequences.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Identification and Characterization of a Male Sterile Rapeseed (Plants (Basel, Switzerland) · 2025Article
- Exploration of the molecular mechanism behind a novel natural genic male-sterile mutation of 1205A in Brassica napus.BMC plant biology · 2025Article
- Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene inG3 (Bethesda, Md.) · 2020Article
- Mapping of the male sterile mutant gene ftms in Brassica rapa L. ssp. pekinensis via BSR-Seq combined with whole-genome resequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in theInternational journal of molecular sciences · 2018Article
- Identification of candidate genes underlying genic male-sterile msc-1 locus via genome resequencing in Capsicum annuum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Fine mapping of the genic male-sterile msTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Comparative Mapping Combined With Map-Based Cloning of theFrontiers in plant science · 2018Article
- Article
- Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus.Scientific reports · 2016Article
- Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.Breeding science · 2016Article
- Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Mapping of BnMs4 and BnRf to a common microsyntenic region of Arabidopsis thaliana chromosome 3 using intron polymorphism markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Review
- A large insertion in bHLH transcription factor BrTT8 resulting in yellow seed coat in Brassica rapa.PloS one · 2012Article
- Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Development and evaluation of single-nucleotide polymorphism markers in allotetraploid rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
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7 authors.
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Abstract
S45AB, a recessive genic male sterile (RGMS) line, originated as a spontaneous mutant in Brassica napus cv. Oro. The genotypes of sterile (S45A) and fertile plants (S45B) are Bnms1ms1ms2ms2 and BnMs1ms1ms2ms2, respectively. In our previous studies, Yi et al. (Theor Appl Genet 113:643-650, 2006) mapped the BnMs1 locus to a region of 0.4 cM, candidates of which have been identified and genetic transformation is in progress. We describe the fine mapping of BnMs2 exploiting amplified fragment length polymorphism (AFLP) and amplified consensus genetic marker (ACGM) methodologies, and the identification of a collinear region probably containing BnMs2 orthologue in Arabidopsis thaliana. A near isogenic line (NIL) population S4516AB which segregated for BnMs2 locus was generated by crossing, allelism testing and repeated full-sib mating. From the survey of 1,024 AFLP primer combinations, 12 tightly linked AFLP markers were obtained and five of them were successfully converted into co-dominant or dominant sequence characterized amplified region (SCAR) markers. A population of 2,650 sterile plants was screened using these markers and a high-resolution map surrounding BnMs2 was constructed. The closest AFLP markers flanking BnMs2 were 0.038 and 0.075 cM away, respectively. Subsequently, an ACGM marker was developed to delimit the BnMs2 locus at an interval of 0.075 cM. We extended marker sequences to perform BlastN searches against the Arabidopsis genome and identified a collinear region containing 68 Arabidopsis genes, in which the orthologue of BnMs2 might be included. We further integrated BnMs2 linked AFLP or SCAR markers to two doubled-haploid (DH) populations derived from the crosses Tapidor x Ningyou7 (Qiu et al., Theor Appl Genet 114:67-80, 2006) and Quantum x No.2127-17 (available in our laboratory), and BnMs2 was mapped on N16. Molecular markers developed from these investigations will facilitate the marker-assisted selection (MAS) of RGMS lines, and the fine map and syntenic region identified will greatly hasten the process of positional cloning of BnMs2 gene.
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