ArticleBiophysical journal1991
Kinetic constants of the acetylcholine (ACh) receptor reaction deduced from the rise in open probability after steps in ACh concentration.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 53 citations in OpenAlex.
- Agonists binding nicotinic receptors elicit specific channel-opening patterns at αγ and αδ sites.The Journal of physiology · 2014Article
- Perspectives on: conformational coupling in ion channels: allosteric coupling in ligand-gated ion channels.The Journal of general physiology · 2012Article
- Unraveling subunit cooperativity in homotetrameric HCN2 channels.Biophysical journal · 2012Article
- Dependence of miniature endplate current on kinetic parameters of acetylcholine receptors activation: a model study.Neurochemical research · 2003Article
- Presynaptic M(2) muscarinic receptors are involved in controlling the kinetics of ACh release at the frog neuromuscular junction.The Journal of physiology · 2001Article
- Evaluation of the number of agonist molecules needed to activate a ligand-gated channel from the current rising phase.Biophysical journal · 2000Article
- Single channel currents at six microsecond resolution elicited by acetylcholine in mouse myoballs.The Journal of physiology · 1998Article
- Depolarization increases the single-channel conductance and the open probability of crayfish glutamate channels.Biophysical journal · 1998Article
- The channel opening rate of adult- and fetal-type mouse muscle nicotinic receptors activated by acetylcholine.The Journal of physiology · 1998Article
- Activation kinetics of glutamate receptor channels from wild-type Drosophila muscle.Pflugers Archiv : European journal of physiology · 1996Article
- Kinetics of homomeric GluR6 glutamate receptor channels.Biophysical journal · 1996Article
- Characterization and molecular reaction scheme of a chloride channel expressed after axotomy in crayfish.Pflugers Archiv : European journal of physiology · 1996Article
- Neurotransmitter discharge and postsynaptic rise times.Biophysical journal · 1996Article
- Acetylcholine-induced currents in acutely dissociated sympathetic neurons from adult hypertensive and normotensive rats have similar properties.Pflugers Archiv : European journal of physiology · 1995Article
- GABAergic inhibition of crayfish deep extensor abdominal muscle exhibits a steep dose-response relationship and a high degree of cooperativity.Pflugers Archiv : European journal of physiology · 1995Article
- Multiphasic desensitization of the GABAA receptor in outside-out patches.Biophysical journal · 1994Article
- Gating kinetics of the quisqualate-sensitive glutamate receptor of locust muscle studied using agonist concentration jumps and computer simulations.Biophysical journal · 1993Article
- A molecular scheme for the reaction between acetylcholine and nicotinic channels.Biophysical journal · 1993Article
- Activation and desensitization of embryonic-like receptor channels in mouse muscle by acetylcholine concentration steps.The Journal of physiology · 1992Article
- Neuromuscular glutamatergic and GABAergic channels.Invertebrate neuroscience : INReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outside-out patches of enzymatically dissociated adult and denervated mouse muscle fibers were superfused repetitively by pulses of acetylcholine (ACh) containing solution. Up to 300 channels opened simultaneously 300 microseconds after the beginning of a 1,000 microM ACh pulse corresponding to a peak current i of almost -1 nA. Single responses to ACh were averaged and the concentration dependence of i and of the rise time tr from 0.1 i to 0.9 i was measured. In adult receptors, i increased proportional to the second to third power of ACh concentration, whereas in embryonic-type receptors it was proportional to the first to the second power. tr increased from approximately 0.3 ms at 1,000 microM ACh to a plateau value of approximately 5 ms for adult and of approximately 10 ms for embryoniclike receptors at concentrations less than 10 microM ACh. The concentration dependence of i and tr was simulated using the standard model of ACh binding with different combinations of rate constants and two and three binding sites for ACh. The calculated curves were compared to the measurements and a set of well fitting rate constants was determined for adult and embryoniclike receptors. Three binding sites for ACh were necessary to fit the dose response for i for adult receptors. A method for deriving rate constants in a model of ACh-receptor interaction is described that avoids analysis of open-closed kinetics of single channels, which in rapid systems, as the ones studied here, are at the limit of the frequency response of the current measurement.
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