ArticleBiophysical journal2007
Second harmonic generation in neurons: electro-optic mechanism of membrane potential sensitivity.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.Biophysical journal · 2026Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- A Modified Oxazolone Dye Dedicated to Spectroscopy and Optoelectronics.The Journal of organic chemistry · 2022Article
- Phagosomal chloride dynamics in the alveolar macrophage.iScience · 2022Article
- Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria.Biophysical journal · 2019Article
- Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.Frontiers in molecular biosciences · 2019Review
- Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level.Nature communications · 2018Article
- Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells.iScience · 2018Article
- Multimodal two-photon imaging using a second harmonic generation-specific dye.Nature communications · 2016Article
- Label-free probe of HIV-1 TAT peptide binding to mimetic membranes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane.Frontiers in molecular neuroscience · 2014Article
- Porphyrins for probing electrical potential across lipid bilayer membranes by second harmonic generation.Angewandte Chemie (International ed. in English) · 2013Article
- Probing the orientational distribution of dyes in membranes through multiphoton microscopy.Biophysical journal · 2012Article
- Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish.Nature protocols · 2012Article
- Label-free imaging of membrane potential using membrane electromotility.Biophysical journal · 2012Article
- Imaging voltage in neurons.Neuron · 2011Review
- Second Harmonic Generation for time-resolved monitoring of membrane pore dynamics subserving electroporation of neurons.Biomedical optics express · 2011Article
- Second-harmonic generation imaging of membrane potential with retinal analogues.Biophysical journal · 2011Article
- Advances in light microscopy for neuroscience.Annual review of neuroscience · 2009Review
- Second-harmonic generation imaging of membrane potential with photon counting.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second harmonic generation (SHG) from membrane-bound chromophores can be used to image membrane potential in neurons. We investigate the biophysical mechanism responsible for the SHG voltage sensitivity of the styryl dye FM 4-64 in pyramidal neurons from mouse neocortical slices. SHG signals are exquisitely sensitive to the polarization of the incident laser light. Using this polarization sensitivity in two complementary approaches, we estimate a approximately 36 degrees tilt angle of the chromophore to the membrane normal. Changes in membrane potential do not affect the polarization of the SHG signal. The voltage response of FM 4-64 is faster than 1 ms and does not reverse sign when imaged at either side of its absorption peak. We conclude that FM 4-64 senses membrane potential through an electro-optic mechanism, without significant chromophore membrane reorientation, redistribution, or spectral shift.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.