ArticleBritish journal of pharmacology2007
Pharmacological activators of AMP-activated protein kinase have different effects on Na+ transport processes across human lung epithelial cells.
Article in British journal of pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Culture with apically applied healthy or disease sputum alters the airway surface liquid proteome and ion transport across human bronchial epithelial cells.American journal of physiology. Cell physiology · 2021Article
- The role of AMPK in regulation of NaJournal of muscle research and cell motility · 2021Review
- AMPK alleviates high uric acid-induced NaExperimental & molecular medicine · 2019Article
- Metformin attenuates the effect of Staphylococcus aureus on airway tight junctions by increasing PKCζ-mediated phosphorylation of occludin.Journal of cellular and molecular medicine · 2019Article
- AMP-Activated Protein Kinase (AMPK)-Dependent Regulation of Renal Transport.International journal of molecular sciences · 2018Review
- BMI-1 extends proliferative potential of human bronchial epithelial cells while retaining their mucociliary differentiation capacity.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- ENaCs as Both Effectors and Regulators of MiRNAs in Lung Epithelial Development and Regeneration.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2017Review
- Lack of Effects of Metformin and AICAR Chronic Infusion on the Development of Hypertension in Dahl Salt-Sensitive Rats.Frontiers in physiology · 2017Article
- Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells.International journal of biological sciences · 2016Review
- LKB1 Tumor Suppressor: Therapeutic Opportunities Knock when LKB1 Is Inactivated.Genes & diseases · 2014Article
- Metformin-stimulated AMPK-α1 promotes microvascular repair in acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- AMP-activated protein kinase (AMPK)-dependent and -independent pathways regulate hypoxic inhibition of transepithelial Na+ transport across human airway epithelial cells.British journal of pharmacology · 2012Article
- Activation of AMP-activated protein kinase stimulates Na+,K+-ATPase activity in skeletal muscle cells.The Journal of biological chemistry · 2012Article
- The gasotransmitter hydrogen sulphide decreases Na⁺ transport across pulmonary epithelial cells.British journal of pharmacology · 2012Article
- Gasotransmitters: novel regulators of epithelial na(+) transport?Frontiers in physiology · 2012Article
- Oxygen regulation of the epithelial Na channel in the collecting duct.American journal of physiology. Renal physiology · 2011Article
- Role of the energy sensor AMP-activated protein kinase in renal physiology and disease.American journal of physiology. Renal physiology · 2010Review
- AMPK controls epithelial Na(+) channels through Nedd4-2 and causes an epithelial phenotype when mutated.Pflugers Archiv : European journal of physiology · 2009Article
- Regulation of epithelial sodium channels by cGMP/PKGII.The Journal of physiology · 2009Article
- Regulation of Cl(-) secretion by AMPK in vivo.Pflugers Archiv : European journal of physiology · 2009Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
background and purposeAMP-activated protein kinase (AMPK) is activated by metformin, phenformin, and the AMP mimetic, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR). We have completed an extensive study of the pharmacological effects of these drugs on AMPK activation, adenine nucleotide concentration, transepithelial amiloride-sensitive (I(amiloride)) and ouabain-sensitive basolateral (I(ouabain)) short circuit current in H441 lung epithelial cells. EXPERIMENTAL APPROACH: H441 cells were grown on permeable filters at air interface. I(amiloride), I(ouabain) and transepithelial resistance were measured in Ussing chambers. AMPK activity was measured as the amount of radiolabelled phosphate transferred to the SAMS peptide. Adenine nucleotide concentration was analysed by reverse phase HPLC and NAD(P)H autofluorescence was measured using confocal microscopy. KEY
resultsPhenformin, AICAR and metformin increased AMPK (alpha1) activity and decreased I(amiloride). The AMPK inhibitor Compound C prevented the action of metformin and AICAR but not phenformin. Phenformin and AICAR decreased I(ouabain) across H441 monolayers and decreased monolayer resistance. The decrease in I(amiloride) was closely related to I(ouabain) with phenformin, but not in AICAR treated monolayers. Metformin and phenformin increased the cellular AMP:ATP ratio but only phenformin and AICAR decreased cellular ATP. CONCLUSIONS AND IMPLICATIONS: Activation of alpha1-AMPK is associated with inhibition of apical amiloride-sensitive Na(+) channels (ENaC), which has important implications for the clinical use of metformin. Additional pharmacological effects evoked by AICAR and phenformin on I(ouabain), with potential secondary effects on apical Na+ conductance, ENaC activity and monolayer resistance, have important consequences for their use as pharmacological activators of AMPK in cell systems where Na+K+ATPase is an important component.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.