ArticleBritish journal of pharmacology2007
High-throughput microscopy must re-invent the microscope rather than speed up its functions.
Article in British journal of pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Random access parallel microscopy.eLife · 2021Article
- Review
- Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology.Applied physics letters · 2013Article
- Lens-free optical tomographic microscope with a large imaging volume on a chip.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Color capable sub-pixel resolving optofluidic microscope and its application to blood cell imaging for malaria diagnosis.PloS one · 2011Article
- The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts.Biomedical microdevices · 2009Article
- A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization.Nucleic acids research · 2009Article
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1 author.
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No grant is acknowledged in the PubMed record.
Abstract
Knowledge gained from the revolutions in genomics and proteomics has helped to identify many of the key molecules involved in cellular signalling. Researchers, both in academia and in the pharmaceutical industry, now screen, at a sub-cellular level, where and when these proteins interact. Fluorescence imaging and molecular labelling combine to provide a powerful tool for real-time functional biochemistry with molecular resolution. However, they traditionally have been work-intensive, required trained personnel, and suffered from low through-put due to sample preparation, loading and handling. The need for speeding up microscopy is apparent from the tremendous complexity of cellular signalling pathways, the inherent biological variability, as well as the possibility that the same molecule plays different roles in different sub-cellular compartments. Research institutes and companies have teamed up to develop imaging cytometers of ever-increasing complexity. However, to truly go high-speed, sub-cellular imaging must free itself from the rigid framework of current microscopes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.