ArticleThe Plant cell2007
Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Evolutionary and Structural Analysis of PP16 in Viridiplantae.International journal of molecular sciences · 2024Article
- Hexosamine biosynthesis and related pathways, protein N-glycosylation and O-GlcNAcylation: their interconnection and role in plants.Frontiers in plant science · 2024Review
- Plasmodesmal endoplasmic reticulum proteins regulate intercellular trafficking of cucumber mosaic virus in Arabidopsis.Journal of experimental botany · 2023Article
- Engineering Macromolecular Trafficking Into the Citrus Vasculature.Frontiers in plant science · 2022Article
- RBP differentiation contributes to selective transmissibility of OPT3 mRNAs.Plant physiology · 2021Article
- Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants.Current opinion in structural biology · 2021Review
- Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.Plant biotechnology (Tokyo, Japan) · 2021Article
- Phosphorylations of the Abutilon Mosaic Virus Movement Protein Affect Its Self-Interaction, Symptom Development, Viral DNA Accumulation, and Host Range.Frontiers in plant science · 2020Article
- Plasmodesmata Conductivity Regulation: A Mechanistic Model.Plants (Basel, Switzerland) · 2019Review
- Article
- Transcriptome and Metabolome Reprogramming in Tomato Plants byFrontiers in physiology · 2019Article
- Phosphorylation coexists with O-GlcNAcylation in a plant virus protein and influences viral infection.Molecular plant pathology · 2018Article
- Article
- Article
- Glycosylation of the nuclear pore.Traffic (Copenhagen, Denmark) · 2014Review
- Overexpression of the pumpkin (Cucurbita maxima) 16 kDa phloem protein CmPP16 increases tolerance to water deficit.Plant signaling & behavior · 2014Article
- O-GlcNAc modification of the coat protein of the potyvirus Plum pox virus enhances viral infection.Virology · 2013Article
- A plasmodesmal glycosyltransferase-like protein.PloS one · 2013Article
- Overexpression of Arabidopsis plasmodesmata germin-like proteins disrupts root growth and development.The Plant cell · 2012Article
- Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.Journal of experimental botany · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, cell-to-cell trafficking of non-cell-autonomous proteins (NCAPs) involves protein-protein interactions, and a role for posttranslational modification has been implicated. In this study, proteins contained in pumpkin (Cucurbita maxima cv Big Max) phloem sap were used as a source of NCAPs to further explore the molecular basis for selective NCAP trafficking. Protein overlay assays and coimmunoprecipitation experiments established that phosphorylation and glycosylation, on both Nicotiana tabacum NON-CELL-AUTONOMOUS PATHWAY PROTEIN1 (Nt-NCAPP1) and the phloem NCAPs, are essential for their interaction. Detailed molecular analysis of a representative phloem NCAP, Cm-PP16-1, identified the specific residues on which glycosylation and phosphorylation must occur for effective binding to NCAPP1. Microinjection studies confirmed that posttranslational modification on these residues is essential for cell-to-cell movement of Cm-PP16-1. Lastly, a glutathione S-transferase (GST)-Cm-PP16-1 fusion protein system was employed to test whether the peptide region spanning these residues was required for cell-to-cell movement. These studies established that a 36-amino acid peptide was sufficient to impart cell-to-cell movement capacity to GST, a normally cell-autonomous protein. These findings are consistent with the hypothesis that a phosphorylation-glycosylation recognition motif functions to control the binding of a specific subset of phloem NCAPs to NCAPP1 and their subsequent transport through plasmodesmata.
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