ArticleProtein science : a publication of the Protein Society2007
Discovery of antibacterial cyclic peptides that inhibit the ClpXP protease.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- De Novo Discovery of Bioactive Cyclic Peptides via Cellular Selection of Genetically Encoded Libraries.Chembiochem : a European journal of chemical biology · 2026Review
- DnaJ and ClpX Are Required for HitRS and HssRS Two-Component System Signaling in Bacillus anthracis.Infection and immunity · 2022Article
- The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultrahigh-Throughput Screening in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Methods for generating and screening libraries of genetically encoded cyclic peptides in drug discovery.Nature reviews. Chemistry · 2020Review
- Review
- Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models.PeerJ · 2018Article
- Ribosomal Synthesis of Natural-Product-Like Bicyclic Peptides in Escherichia coli.Chembiochem : a European journal of chemical biology · 2015Article
- Ribosomal Synthesis of Macrocyclic Peptides in Vitro and in Vivo Mediated by Genetically Encoded Aminothiol Unnatural Amino Acids.ACS chemical biology · 2015Article
- Identification of inhibitors of a bacterial sigma factor using a new high-throughput screening assay.Antimicrobial agents and chemotherapy · 2015Article
- Cell-based assay to identify inhibitors of the Hfq-sRNA regulatory pathway.Antimicrobial agents and chemotherapy · 2014Article
- Antibacterial activity of and resistance to small molecule inhibitors of the ClpP peptidase.ACS chemical biology · 2013Article
- Pharmacological inhibition of the ClpXP protease increases bacterial susceptibility to host cathelicidin antimicrobial peptides and cell envelope-active antibiotics.Antimicrobial agents and chemotherapy · 2012Article
- Validation of the essential ClpP protease in Mycobacterium tuberculosis as a novel drug target.Journal of bacteriology · 2012Article
- Evolution of cyclic peptide protease inhibitors.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Challenges of antibacterial discovery.Clinical microbiology reviews · 2011Review
- Biological activities of natural and engineered cyclotides, a novel molecular scaffold for peptide-based therapeutics.Current molecular pharmacology · 2010Review
- Synthetic biology approaches in drug discovery and pharmaceutical biotechnology.Applied microbiology and biotechnology · 2010Review
- "Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides.Advanced drug delivery reviews · 2009Review
- Rapid selection of cyclic peptides that reduce alpha-synuclein toxicity in yeast and animal models.Nature chemical biology · 2009Article
- Transcription of clpP is enhanced by a unique tandem repeat sequence in Streptococcus mutans.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A method to rapidly screen libraries of cyclic peptides in vivo for molecules with biological activity has been developed and used to isolate cyclic peptide inhibitors of the ClpXP protease. Fluorescence activated cell sorting was used in conjunction with a fluorescent reporter to isolate cyclic peptides that inhibit the proteolysis of tmRNA-tagged proteins in Escherichia coli. Inhibitors shared little sequence similarity and interfered with unexpected steps in the ClpXP mechanism in vitro. One cyclic peptide, IXP1, inhibited the degradation of unrelated ClpXP substrates and has bactericidal activity when added to growing cultures of Caulobacter crescentus, a model organism that requires ClpXP activity for viability. The screen used here could be adapted to identify cyclic peptide inhibitors of any enzyme that can be expressed in E. coli in conjunction with a fluorescent reporter.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.